Molecular characterization and functional analysis of USP-1 by RNA interference in the Asian gypsy moth Lymantria dispar
Molecular characterization and functional analysis of USP-1 by RNA interference in the Asian gypsy moth Lymantria dispar
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亚洲舞毒蛾 USP-1 RNA 干扰的分子表征和功能分析
DOI：
10.1007/s11676-019-00944-7
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发表时间：
2019-04
期刊：
影响因子：
--
通讯作者：
马岩
中科院分区：
文献类型：
--
作者：
马玲;问荣荣;赵清泉;王步勇;马岩
蜕皮激素在调节节肢动物蜕皮、变态、繁殖和滞育等多种生理过程中起着重要作用。蜕皮甾类通过与两种核受体的异二聚体复合物结合来介导反应：蜕皮激素受体和超气门（USP）。为了研究USP在亚洲舞毒蛾发育中的作用，从舞毒蛾的转录组中获得了USP cDNA。dispar和PCR验证。对L. dispar USP-1（LdUSP-1）在三龄幼虫不同发育阶段和随时间的变化以及在三龄幼虫期间分离的不同组织中的差异。然后进行dispar。LdUSP-1的转录水平在蜕皮后的三龄幼虫和成年雄性中在72小时之前相对较高。利用RNA干扰技术敲低LdUSP-1基因在三龄幼虫中的表达，分析了LdUSP-1基因在蜕皮中的作用。LdUSP-1的沉默显著下调了蜕皮激素诱导基因E75和万圣节基因Sad的转录水平。此外，LdUSP-1敲低后，三龄阶段的持续时间略有缩短，幼虫死亡率增加。
Ecdysteroids play an important role in regulating diverse physiological processes in arthropods, such as molting, metamorphosis, reproduction and diapause. Ecdysteroids mediate the response by binding to a heterodimeric complex of two nuclear receptors: the ecdysone receptor and the ultraspiracle (USP). To investigate the role of USP in development of the Asian gypsy moth (Lymantria dispar), a USP cDNA was obtained from the transcriptome of L. dispar and verified by PCR. In-depth profiling of transcript levels of L. dispar USP-1 (LdUSP-1) at different developmental stages and over time in third-instar larvae and different tissues isolated during the third-instar stage of L. dispar was then carried out. Transcript levels of LdUSP-1 were relatively high before 72 h in the third-instar larvae after ecdysis and in the adult male. The function of LdUSP-1 in molting was analyzed by knockdown of LdUSP-1 in third instar larvae using RNA interference. Silencing of LdUSP-1 significantly downregulated the transcript level of E75, an ecdysone-inducible gene, and of Sad, a Halloween gene. In addition, the duration of the third-instar stage was slightly shortened and larval mortality increased after the LdUSP-1 knockdown.
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DOI：
10.1016/b0-44-451924-6/00041-7
发表时间：
2005
期刊：
--
影响因子：
--
作者：
S. Sakurai
通讯作者：
S. Sakurai
影响因子：
4.6
作者：
B. L. Hall;C. Thummel
通讯作者：
B. L. Hall;C. Thummel
影响因子：
2.7
作者：
Sheng-fu Wang;Chao Li;Jinsong Zhu;K. Miura;R. Miksicek;A. Raikhel
通讯作者：
Sheng-fu Wang;Chao Li;Jinsong Zhu;K. Miura;R. Miksicek;A. Raikhel
影响因子：
3.6
作者：
P. Verma;M. G. Tapadia
通讯作者：
P. Verma;M. G. Tapadia
影响因子：
4.1
作者：
H. M. Krans
通讯作者：
H. M. Krans