Latex agglutination test for detection of Candida antigen in patients with disseminated disease
Latex agglutination test for detection of Candida antigen in patients with disseminated disease
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乳胶凝集试验检测播散性疾病患者念珠菌抗原
DOI：
10.1007/bf02001577
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
M. Price
中科院分区：
文献类型：
--
作者：
L. Gentry;I. D. Wilkinson;Alfred Lea;M. Price
已设计出一种乳胶凝集试验，可用于检测由白色念珠菌、热带念珠菌和近平假丝酵母菌引起的系统性感染患者的循环抗原。乳胶用白念珠菌全热灭活芽胞免疫的兔血清致敏。该血清的活性成分为免疫球蛋白。对照乳胶用于区分非特异性凝集，用相同稀释度的兔血清致敏，不抗念珠菌。测试了来自多个患者组的血清。虽然100名正常对照组中没有一人的⩾抗原滴度为1：4，但33名有记录的播散性念珠菌感染患者中有30人的抗原滴度在1：4至1：32之间。33例中有2例假阴性，1例引起非特异性凝集。在所有经过抗真菌治疗后康复的患者中，抗原水平恢复到正常对照组的范围内。
A latex agglutination test has been devised which allows detection of a circulating antigen in patients with systemic infection due toCandida albicans, Candida tropicalis andCandida parapsilosis. Latex is sensitized with serum from rabbits immunized with whole heat killedCandida albicans blastoconidia. The active component of this serum is IgG. Control latex, used to differentiate non-specific agglutination, is sensitized with the same dilution of serum from a rabbit without antibody toCandida species. Sera from a number of patient groups were tested. While none of the hundred normal controls had an antigen titer of ⩾ 1∶4, 30 of 33 patients with documented disseminated candida infection had antigen titers of 1∶4 to 1∶32. Two of the 33 gave false negative results, and one caused nonspecific agglutination. In all patients who recovered after antifungal therapy antigen levels returned to within the range found in normal controls.