FISH Karyotype of 85 Common Wheat Cultivars/Lines Displayed by ND-FISH Using Oligonucleotide Probes
FISH Karyotype of 85 Common Wheat Cultivars/Lines Displayed by ND-FISH Using Oligonucleotide Probes
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使用寡核苷酸探针通过 ND-FISH 显示 85 个常见小麦品种/品系的 FISH 核型
DOI：
10.1556/0806.45.2017.049
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发表时间：
2017-12-01
影响因子：
1.6
通讯作者：
Tang, Z. X.
中科院分区：
文献类型：
--
作者：
Jiang, M.;Xaio, Z. Q.;Tang, Z. X.
荧光原位杂交（FISH）可以揭示染色体的微小结构差异。基于FISH模式的普通小麦（Triticum aestivum L.）核型研究报道较少。本研究采用非变性FISH （ND-FISH）技术，以Oligo-pSc119.2-1、Oligo-pTa535-1和（AAG）(6)为探针，对85种普通小麦（包括83种小麦-黑麦1RS）的染色体结构进行了研究。1个bl易位品种/系，1个小麦1RS。al易位品种Amigo与中国春（CS）在1A、2A、3A、4A、5A、6A和7A染色体上分别观察到2、3、2、3、6、3和4种结构类型。分别检测到2B、3B、4B、5B、6B和7B的2、8、2、2、4和6种染色体类型。Amigo和CS的1B染色体结构不同。分别区分出1D、2D、3D和5D染色体结构的5种、2种、2种和2种。1RS多态性。1BL、4D、6D、7D染色体未检出。染色体1A(I)、2A(I)、3A(I)、4A(I)、5A（III）、6A(I)、7A（III）、2B(I)、3B(V)、4B(I)、5B（II）、6B（III）、7B(I)、1D（IV）、2D(I)、3D(I)和5D（II）在85个小麦品种/系中出现频率较高。85个小麦品种/品系各有一个独特的核型。阿米戈是一个复杂的易位品种。本研究构建的小麦染色体FISH核型为今后分析小麦遗传资源提供参考，有助于了解小麦染色体的结构变异。此外，本研究结果表明，寡核苷酸探针和ND-FISH技术可以用于小麦品种的个体鉴定。
Fluorescence in situ hybridization (FISH) can reveal minor structural differences of chromosomes. The karyotype of common wheat (Triticum aestivum L.) based on FISH pattern is seldom reported. In this study, non-denaturing FISH (ND-FISH) using Oligo-pSc119.2-1, Oligo-pTa535-1 and (AAG)(6) as probes was used to investigate the chromosomal structure of 85 common wheat including 83 wheat-rye 1RS.1BL translocation cultivars/lines, a wheatrye 1RS.1AL translocation cultivar Amigo and Chinese Spring (CS). Two, three, two, three, six, three and four structural types respectively for 1A, 2A, 3A, 4A, 5A, 6A and 7A chromosomes were observed. Two, eight, two, two, four and six types of chromosome for 2B, 3B, 4B, 5B, 6B and 7B were respectively detected. The structure of 1B chromosomes in Amigo and CS is different. Five, two, two and two types of chromosomal structure respectively for 1D, 2D, 3D and 5D were distinguished. Polymorphisms of 1RS. 1BL, 4D, 6D and 7D chromosomes were not detected. Chromosomes 1A(I), 2A(I), 3A(I), 4A(I), 5A(III), 6A(I), 7A(III), 2B(I), 3B(V), 4B(I), 5B(II), 6B(III), 7B(I), 1D(IV), 2D(I), 3D(I) and 5D(II) appeared in these 85 wheat cultivars/lines at high frequency. Each of the 85 wheat cultivars/lines has a unique karyotype. Amigo is a complex translocation cultivar. The FISH karyotype of wheat chromosomes built in this study provide a reference for the future analyzing wheat genetic stocks and help to learn structural variations of wheat chromosomes. In addition, the results in this study indicate that oligonucleotide probes and ND-FISH technology can be used to identify individual wheat cultivar.