DROSOPHILA SPECTRIN - THE MEMBRANE SKELETON DURING EMBRYOGENESIS
DROSOPHILA SPECTRIN - THE MEMBRANE SKELETON DURING EMBRYOGENESIS
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DOI：
10.1083/jcb.108.5.1697
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发表时间：
1989-05-01
影响因子：
7.8
通讯作者：
BRANTON, D
中科院分区：
文献类型：
--
作者：
PESACRETA, TC;BYERS, TJ;BRANTON, D
用亲和纯化的多克隆或单抗免疫荧光法测定果蝇胚胎中α-血影蛋白的分布。在早期发育过程中，血影蛋白主要集中在细胞膜内表面附近、合体细胞核周围的细胞质孤岛中，在较低浓度时，集中分布在前胚层胚胎的其余细胞质中。随着胚胎发育的进行，血影蛋白的分布随着迁移的核向胚胎表面转移，因此，在核周期9，更大比例的血影蛋白集中在质膜附近。在核周期9和10期间，当细胞核到达细胞表面时，质膜相关的血影蛋白集中在体细胞核上方的帽中。在合体胚胎期的有丝分裂过程中，光影蛋白帽在间期和前期伸长，并分为中期和后期进程。在细胞化过程中，血影蛋白的浓度区域似乎发生了变化：血影蛋白在生长的沟道附近增加，而在胚胎表面相应减少。在皱纹生长的最后阶段，血影蛋白浓度的变化是相反的：在皱纹通道附近，血影蛋白减少，同时在胚胎表面增加。在原肠胚中，鬼影蛋白在胚胎表面附近聚集，尤其是在形成羊水直肠突内陷和头沟时。在胚带伸长阶段，胚胎中的血影蛋白总量显著增加，并均匀分布在几乎所有细胞类型的质膜上。幽灵蛋白水平最高的是呼吸道细胞；最低水平的是形成肠道的部分。幽灵蛋白定位的空间和时间变化表明，该蛋白在稳定而不是启动胚胎结构组织的变化中发挥作用。
The distribution of alpha-spectrin in Drosophila embryos was determined by immunofluorescence using affinity-purified polyclonal or monoclonal antibodies. During early development, spectrin is concentrated near the inner surface of the plasma membrane, in cytoplasmic islands around the syncytial nuclei, and, at lower concentrations, throughout the remainder of the cytoplasm of preblastoderm embryos. As embryogenesis proceeds, the distribution of spectrin shifts with the migrating nuclei toward the embryo surface so that, by nuclear cycle 9, a larger proportion of the spectrin is concentrated near the plasma membrane. During nuclear cycles 9 and 10, as the nuclei reach the cell surface, the plasma membrane-associated spectrin becomes concentrated into caps above the somatic nuclei. Concurrent with the mitotic events of the syncytial blastoderm period, the spectrin caps elongate at interphase and prophase, and divide as metaphase and anaphase progress. During cellularization, the regions of spectrin concentration appear to shift: spectrin increases near the growing furrow canal and concomitantly decreases at the embryo surface. In the final phase of furrow growth, the shift in spectrin concentration is reversed: spectrin decreases near the furrow canal and concomitantly increases at the embryo surface. In gastrulae, spectrin accumulates near the embryo surface, especially at the forming amnioproctodeal invagination and cephalic furrow. During the germband elongation stage, the total amount of spectrin in the embryo increases significantly and becomes uniformly distributed at the plasma membrane of almost at cell types. The highest levels of spectrin are in the respiratory tract cells; the lowest levels are in parts of the forming gut. The spatial and temporal changes in spectrin localization suggest that this protein plays a role in stabilizing rather than initiating changes in structural organization in the embryo.