Biofilm in the sediment phase of a sanitary gravity sewer
Biofilm in the sediment phase of a sanitary gravity sewer
复制标题
DOI：
10.1016/s0043-1354(03)00083-6
复制
发表时间：
2003-06-01
期刊：
影响因子：
12.8
通讯作者：
Hung, JC
中科院分区：
文献类型：
--
作者：
Chen, GH;Leung, DHW;Hung, JC
本文研究了长1.5 km、直径540 mm的水泥管道中泥沙相的微生物活性。对沉积物样品的扫描电镜检查和基本成分分析表明，沉积物表面存在生物膜层。dna染色技术的细菌计数结果显示，这一层的细菌数量为2.1 × 10（11）细胞g（-1）干重量，与活性污泥的细菌数量接近。污水生物膜中ATP含量较高，表明污水生物膜具有活性。在整个1.5 km的污水段，生物膜活性基本保持在同一水平。实验室尺度沉积物吸氧通量（SOUF）试验表明，沉积物相上方的剪切流速线性增加了SOUF，在下水管道25°c处估计剪切流速为0.055 m s(-1)，平均流速为1.5 m s(-1)，平均水深为220 mm的情况下，确定其潜在值为32 g O-2 m(-2) day（-1）。如此高的SOUF值进一步证实了活性下水道生物膜的存在。2003爱思唯尔科学有限公司版权所有。
Microbial activity of the sediment phase in a 1.5-km-long concrete sewer section with a cement pipe in a 540-mm diameter was investigated in this paper. SEM examinations and elementary composition analyses of the sediment samples have identified the presence of a biofilm layer at the sediment surface. Bacterial counting results with a DNA-staining technique have revealed that the amount of bacteria in this layer was 2.1 x 10(11) cell g(-1) dry wt, which is close to that of activated sludge. ATP content in the sewer biofilm was found relatively high, demonstrating that the sewer biofilm is active. Throughout the entire 1.5-km sewer section, the biofilm activity was maintained at almost the same level. Lab-scale sediment oxygen uptake flux (SOUF) tests showed that the shear flow velocity above the sediment phase linearly increases the SOUF, which of the potential value was determined to be 32 g O-2 m(-2) day(-1) at an estimated shear flow velocity of 0.055 m s(-1) at 25degreesC in the sewer line, provided that the mean flow velocity was 1.5 m s(-1), and the mean water depth was 220 mm. Such a high SOUF value further endorsed the existence of the active sewer biofilm. (C) 2003 Elsevier Science Ltd. All rights reserved.