Refined methodology for implantation of a head fixation device and chronic recording chambers in non-human primates
Refined methodology for implantation of a head fixation device and chronic recording chambers in non-human primates
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DOI：
10.1016/j.jneumeth.2013.07.015
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发表时间：
2013-10-15
影响因子：
3
通讯作者：
Rouiller, E. M.
中科院分区：
文献类型：
--
作者：
Lanz, F.;Lanz, X.;Rouiller, E. M.
本研究旨在开发一种新的策略，设计和锚定制安装的植入物，包括头部固定装置和慢性记录室，在成年猕猴的头骨。这是在没有使用牙科树脂或骨科骨水泥的情况下完成的，因为这些固定模式对骨骼产生不利影响。植入物由钛或tekapeek制成，并用钛螺钉固定在颅骨上。在进行电生理学研究前几个月，首先对两只成年猕猴植入头部固定装置，以实现最佳骨整合，包括植入物底板上方的骨生长。在第二步中，将慢性记录室植入感兴趣的大脑区域上方。本研究提出了两种植入物的两种原始方法。首先，基于对猴子的CT扫描，以3D打印的形式获得了头骨的塑料复制品，用于精确地塑造和定位两个植入物。这将确保与头骨表面完美匹配。其次，植入物与骨接触的部分涂有羟基磷灰石，呈现与天然骨的化学相似性，从而促进良好的骨整合。此处使用的植入物的寿命为：股骨头固定装置为4年，慢性腔室为1.5年。没有不良事件，日常护理很容易。这清楚地证明了本植入策略是成功的，并且对动物引起的不适较少。(C)2013作者Elsevier B.V.出版，保留所有权利。
The present study was aimed at developing a new strategy to design and anchor custom-fitted implants, consisting of a head fixation device and a chronic recording chamber, on the skull of adult macaque monkeys. This was done without the use of dental resin or orthopedic cement, as these modes of fixation exert a detrimental effect on the bone. The implants were made of titanium or tekapeek and anchored to the skull with titanium screws. Two adult macaque monkeys were initially implanted with the head fixation device several months previous to electrophysiological investigation, to allow optimal osseous-integration, including growth of the bone above the implant's footplate. In a second step, the chronic recording chamber was implanted above the brain region of interest. The present study proposes two original approaches for both implants. First, based on a CT scan of the monkey, a plastic replicate of the skull was obtained in the form of a 3D print, used to accurately shape and position the two implants. This would ensure a perfect match with the skull surface. Second, the part of the implants in contact with the bone was coated with hydroxyapatite, presenting chemical similarity to natural bone, thus promoting excellent osseous-integration. The longevity of the implants used here was 4 years for the head fixation device and 1.5 years for the chronic chamber. There were no adverse events and daily care was easy. This is clear evidence that the present implanting strategy was successful and provokes less discomfort to the animals. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved.