Early testicular response to intraperitoneal administration of ethane dimethanesulphonate (EDS) in the goat (Capra hircus).
Early testicular response to intraperitoneal administration of ethane dimethanesulphonate (EDS) in the goat (Capra hircus).
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山羊（Capra hircus）腹腔注射二甲磺酸乙烷（EDS）后睾丸的早期反应。
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
G. Werner
中科院分区：
文献类型：
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作者：
D. Onyango;E. Wang;D. Oduor‐Okelo;G. Werner
应用光镜和电镜观察了一次性腹腔注射乙烷二甲烷磺酸盐（EDS）后山羊睾丸的早期形态学变化。该化合物以两种剂量水平给药：75 mg/kg和25 mg/kg。前者因毒性导致一些动物死亡，而后者对动物没有明显的毒性作用。两种剂量水平下的睾丸效应相似。给药后6天，间质细胞对EDS激发不敏感，但精子发生明显中断。这些Leydig细胞表现出卵圆形或不规则圆形的核，丰富的细胞质含有球形，卵圆形或细长的线粒体和优势的滑面内质网的典型的正常细胞。脂滴罕见。曲细精管中的生殖细胞和支持细胞变性。生殖细胞的变化包括：精原细胞变性、细线期精母细胞染色质浓缩和染色质重组失败，导致细胞分裂末期（生精周期第4阶段）细胞中形成团块。初级精母细胞的核膜显示出明显的不规则性，细胞大小总体上减少。在第1、2和5期精子细胞的发育过程中，染色质在周围重新分布，通常导致沿核膜的厚边沿着形成，并留下一个苍白的核质。还观察到成熟期精子细胞在第2阶段小管中的伴随保留。支持细胞表现出广泛积累的胞质内小泡，模糊其余的细胞器。上皮细胞内也出现细胞间空泡。结果表明，虽然EDS对山羊Leydig细胞没有任何影响，但它会严重破坏精子发生过程。此外，根据结果得出结论，该物种的最佳剂量为25 mg/kg。
Early morphological changes in the goat testis after a single intraperitoneal injection of ethane dimethanesulphonate (EDS) were investigated using both light and electron microscopy. The compound was administered at two dose levels: 75 mg/kg and 25 mg/kg. While the former resulted in some deaths due to toxicity, the latter had no noticeable toxic effects on the animals. The testicular effects at both dose levels were similar. Six (6) days post-treatment, Leydig cells were refractory to EDS challenge but there was a marked disruption of spermatogenesis. These Leydig cells exhibited ovoid or irregularly round nuclei, abundant cytoplasm containing spherical, ovoid or elongate mitochondria and a preponderance of smooth endoplasmic reticulum typical of the normal cells. Lipid droplets were rare. In the seminiferous tubules germ and Sertoli cell degeneration was observed. Changes in the germ cells included: spermatogonial degeneration, condensed chromatin in leptotene spermatocytes and failure of chromatin re-organization resulting in the formation of clumps in the cells at the telophase stage of cell division (stage 4 of the seminiferous cycle). The nuclear envelope of primary spermatocytes showed marked irregularity and there was an overall reduction in cell size. There was peripheral re-distribution of chromatin in developing spermatids of stages 1, 2 and 5, often resulting in thick margination along the nucleolemma and leaving a pale nucleoplasm. An accompanying retention of maturation phase spermatids in stage 2 tubules was also observed. Sertoli cells exhibited extensive accumulation of intracytoplasmic vesicles, obscuring the rest of the organelles. Intercellular vacuoles also occurred within the epithelium. The results suggest that while EDS does not have any effect on goat Leydig cells, it causes severe disruption of the spermatogenic process. Furthermore, it is concluded from the results that the optimum dose in this species is 25 mg/kg.