Measuring zinc in living cells. A new generation of sensitive and selective fluorescent probes
Measuring zinc in living cells. A new generation of sensitive and selective fluorescent probes
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DOI：
10.1016/s0143-4160(02)00053-2
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发表时间：
2002-05-01
期刊：
影响因子：
4
通讯作者：
Weiss, JH
中科院分区：
文献类型：
--
作者：
Gee, KR;Zhou, ZL;Weiss, JH
合成了一种新型的锌离子荧光指示剂，它对锌离子具有纳摩尔到微摩尔的亲和力，波长从紫外光到远红光。紫外光激发的指示剂是比率指示剂。可见波长指示剂是非比率的，表现出大的、与pH无关的荧光随着锌浓度的增加而增加，对生理上相关的钙离子浓度几乎不敏感。在神经细胞和非神经细胞培养中的实验表明，新的指示剂在转化为细胞渗透形式后，保持了对锌的敏感性和选择性。(C)2002爱思唯尔科学有限公司。保留所有权利。
New fluorescent indicators with nanomolar to micromolar affinities for Zn2+ have been synthesized in wavelengths from UV to the far red. The UV light-excited indicators are ratiometric. The visible wavelength indicators are non-ratiometric and exhibit large and pH-independent fluorescence increases with increasing zinc concentrations, with little to no sensitivity to physiologically relevant Ca2+ concentrations. Experiments in neuronal and non-neuronal cell cultures show the new indicators to retain their sensitivity to and selectivity for zinc after conversion to cell-permeable forms. (C) 2002 Elsevier Science Ltd. All rights reserved.