Immunocytochemical localization of an allatotropin in developmental stages of Heliothis virescens and Apis mellifera
Immunocytochemical localization of an allatotropin in developmental stages of Heliothis virescens and Apis mellifera
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DOI：
10.1016/j.jinsphys.2004.12.005
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发表时间：
2005-04-01
影响因子：
2.2
通讯作者：
Rachinsky, A
中科院分区：
文献类型：
--
作者：
Glasscock, JM;Mizoguchi, A;Rachinsky, A
咽侧体的保幼激素生物合成受称为allatotropins的刺激性神经肽和称为allatostatins的抑制性神经肽调节。本研究定位Manduca sexta allatotropin样物质的发育阶段的夜蛾Heliothis virescens和蜜蜂蜜蜂意大利蜜蜂。使用荧光标记抗体和酶偶联抗体的免疫细胞化学方法被用来染色这两个物种的中枢神经组织。H. virescens含有M. sexta allatotropin（Manse-AT）样物质在整个幼虫发育过程中始终存在。Manse-AT免疫反应细胞体在中枢神经系统的分布模式持续从一个幼虫龄期到下一个。将讨论幼虫Manse-AT的分布模式不同的成年人。脑和食管下神经节中含AT神经元的总数在幼虫发育过程中逐渐增加，而胸、腹神经节中含AT神经元的数量保持不变。Manse-AT免疫反应阳性细胞仅见于少数末龄晚期幼虫（前蛹）的脑中。Manse-AT样物质存在于大脑间部的一组6-8个细胞中。但是，我们没有找到。末龄早期幼虫脑中的任何Manse-AT样物质，其咽侧体（CA）对Manse-AT的体外刺激比预蛹CA更敏感。(c)2005爱思唯尔有限公司保留所有权利。
Juvenile hormone biosynthesis by the corpora allata is regulated by stimulatory neuropeptides called allatotropins and inhibitory neuropeptides called allatostatins. This study localized Manduca sexta allatotropin-like material in developmental stages of the noctuid moth Heliothis virescens and the honeybee Apis mellifera. Immunocytochemical methods using both fluorescence-tagged antibodies and enzyme-coupled antibodies were used to stain the central nervous tissue of both species. H. virescens contains M. sexta allatotropin (Manse-AT)-like material consistently throughout larval development. The distribution patterns of Manse-AT immunoreactive cell bodies in the CNS persisted from one larval instar to the next. It will be discussed how larval Manse-AT distribution patterns differed from those in adults. The total number of AT-containing cells in brain and subesophageal ganglion gradually increased during larval development, whereas in the thoracic and abdominal ganglia, the number of AT-containing neurons remained constant: In the honeybee A. mellifera, Manse-AT immunoreactive cells were only found in a few brains from late last instar larvae (prepupae). Manse-AT-like material was present in a group of 6-8 cells in the pars intercerebralis. However, we did not find. any Manse-AT-like material in brains of early last instar larvae, whose corpora allata (CA) are more sensitive to in vitro stimulation by Manse-AT than prepupal CA. (c) 2005 Elsevier Ltd. All rights reserved.