Proteoliposomes as energy transferring nanomaterials: enhancing the spectral range of light-harvesting proteins using lipid-linked chromophores
Proteoliposomes as energy transferring nanomaterials: enhancing the spectral range of light-harvesting proteins using lipid-linked chromophores
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DOI：
10.1101/609255
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发表时间：
2019-04
期刊：
影响因子：
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通讯作者：
Ashley M. Hancock;Sophie A. Meredith;S. Connell;L. Jeuken;Peter G. Adams
中科院分区：
文献类型：
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作者：
Ashley M. Hancock;Sophie A. Meredith;S. Connell;L. Jeuken;Peter G. Adams
生物学提供了一套光学活性纳米材料，其形式为“捕光”蛋白质-叶绿素复合物，然而，这些材料具有缺点，包括其有限的光谱范围。我们报告了模型脂质膜（蛋白脂质体）的产生，将光合蛋白光捕获复合物II（LHCII）和脂质束缚的德克萨斯红（TR）发色团作为一种“生物混合”能量转移纳米材料。由于TR发色团的高效能量转移（高达94%），该蛋白质的有效光谱范围得到增强，从而使LHCII荧光显着增加（高达3倍）。我们的自组装过程提供了优异的模块性，允许掺入一系列浓度的能量供体（TR）和受体（LHCII），允许根据需要调节能量转移效率（埃特）和LHCII荧光。荧光寿命成像显微镜（FLIM）提供了埃特的单蛋白脂质体水平定量，揭示了群体内的分布，并证明功能性保持在表面上。我们的基于膜的系统作为一个可控的光捕获纳米材料与潜在的应用，作为薄膜的光敏器件。目录图
Biology provides a suite of optically-active nanomaterials in the form of “light harvesting” protein-chlorophyll complexes, however, these have drawbacks including their limited spectral range. We report the generation of model lipid membranes (proteoliposomes) incorporating the photosynthetic protein Light-Harvesting Complex II (LHCII) and lipid-tethered Texas Red (TR) chromophores that act as a “bio-hybrid” energy transferring nanomaterial. The effective spectral range of the protein is enhanced due to highly efficient energy transfer from the TR chromophores (up to 94%), producing a marked increase in LHCII fluorescence (up to 3x). Our self-assembly procedure offers excellent modularity allowing the incorporation of a range of concentrations of energy donors (TR) and acceptors (LHCII), allowing the energy transfer efficiency (ETE) and LHCII fluorescence to be tuned as desired. Fluorescence Lifetime Imaging Microscopy (FLIM) provides single-proteoliposome-level quantification of ETE, revealing distributions within the population and proving that functionality is maintained on a surface. Our membrane-based system acts as a controllable light harvesting nanomaterial with potential applications as thin films in photo-active devices. Table of Contents Figure