AN ULTRASTRUCTURAL-STUDY ON THE ROLE OF KUPFFER CELLS IN THE PROCESS OF INFECTION BY PLASMODIUM-BERGHEI SPOROZOITES IN RATS
AN ULTRASTRUCTURAL-STUDY ON THE ROLE OF KUPFFER CELLS IN THE PROCESS OF INFECTION BY PLASMODIUM-BERGHEI SPOROZOITES IN RATS
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DOI：
10.1017/s003118200005040x
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发表时间：
1983-01-01
期刊：
影响因子：
2.4
通讯作者：
MEUWISSEN, JHET
中科院分区：
文献类型：
--
作者：
MEIS, JFGM;VERHAVE, JP;MEUWISSEN, JHET
应用透射电镜观察了伯氏疟原虫子孢子与大鼠肝脏枯否细胞的相互作用。接种后10和15分钟，子孢子在肝窦和枯否细胞的内吞空泡内都是游离的。后者在吞噬子孢子、细菌和红细胞方面非常活跃。子孢子在内吞空泡内保持其完整性，未观察到溶酶体消化的迹象。接种后1小时内胞吞空泡内看到的子孢子形态上仍然完整，虽然刷毛包被的囊泡与空泡膜融合。枯否细胞可将子孢子运输到Disse间隙和邻近的肝细胞。未见子孢子穿透内皮细胞或其狭窄的窗孔。显然，库普弗细胞通道，而不是窦状隙衬里的间隙，代表子孢子绕过内皮屏障的正常途径。红细胞外型的定位更容易通过使用布朗挪威大鼠，其中有更多的寄生虫比Wistar大鼠。寄生虫的分布主要在肝组织腺泡的门静脉周围。
The interactions in vivo between P. berghei sporozoites and Kupffer cells in rat livers were studied by transmission electron microscopy. By 10 and 15 min after inoculation, sporozoites were both free in the liver sinusoids and inside endocytotic vacuoles of the Kupffer cells. The latter cells were very active in phagocytosing sporozoites, bacteria and red blood cells. The sporozoites retained their integrity inside the endocytotic vacuoles and no signs of lysosomal digestion were observed. Sporozoites seen within endocytotic vacuoles 1 h after inoculation were still morphologically intact, although bristle-coated vesicles fused with the vacuole membrane. Kupffer cells may transport sporozoites towards the space of Disse and adjacent hepatocytes. No sporozoites were seen to penetrate an endothelial cell or its narrow fenestrae. Apparently, Kupffer cell passage, rather than gaps in the sinusoidal lining, represents the normal route that sporozoites take to circumvent the endothelial barrier. The localization of exoerythrocytic forms was made easier by the use of Brown Norway rats in which many more parasites develop than in the Wistar rats. The distribution pattern of the parasites was mainly around the periportal zones of the acini of liver tissue.