Hepatoprotective effects of baicalein against CCl4-induced acute liver injury in mice
Hepatoprotective effects of baicalein against CCl4-induced acute liver injury in mice
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DOI：
10.3748/wjg.v18.i45.6605
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发表时间：
2012-12-07
影响因子：
4.3
通讯作者：
Zhu, Run-Zhi
中科院分区：
文献类型：
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作者：
Huang, Hai-Li;Wang, Ya-Jing;Zhu, Run-Zhi
目的：目的：观察黄芩素对四氯化碳（CCl 4）诱导的小鼠肝损伤的保护作用。方法：小鼠腹腔注射CCl 4后，灌胃黄芩素，治疗组给予黄芩素，2次/d，共4d。通过肝组织病理学和血清谷丙转氨酶、谷草转氨酶的测定，直接评价黄芩素的抗炎作用。用增殖细胞核抗原检测黄芩素对肝细胞增殖的影响。血清白细胞介素（IL）-6、IL-1 β和肿瘤坏死因子-α采用酶联免疫吸附试验检测TNF-α水平，并检测肝组织中IL-6、TNF-α、转化生长因子-α采用实时定量聚合酶链反应（Real-time PCR）检测TGF-α、肝细胞生长因子（HGF）和表皮生长因子（EGF）基因表达。CCl 4诱导的急性肝衰竭模型在黄芩苷处理的小鼠中提供了生存益处。结果表明，黄芩素处理后12 h内，IL-6和TNF-α的mRNA水平显著升高，但在24、48和72 h，IL-6和TNF-α的表达水平均低于对照组。黄芩素组TGF-α、HGF和EGF的表达在12、24、18和72 h均明显增强。结论：黄芩素可能是治疗急性肝损伤的一种有效药物。黄芩素通过调节TNF-α和IL-6介导的途径促进肝再生。(C)2012年百世登。All rights reserved.
AIM: To investigate the hepatoprotective effect of baicalein against carbon tetrachloride (CCl4)-induced liver damage in mice.METHODS: Mice were orally administered with baicalein after CCl4 injection, and therapeutic baicalein was given twice a day for 4 d. The anti-inflammation effects of baicalein were assessed directly by hepatic histology and serum alanine aminotranferease and aspartate aminotransferase measurement. Proliferating cell nuclear antigen was used to evaluate the effect of baicalein in promoting hepatocyte proliferation. Serum interleukin (IL)-6, IL-1 beta and tumor necrosis factor-alpha (TNF-alpha) levels were measured by enzyme-linked immunosorbent assay and liver IL-6, TNF-alpha, transforming growth factor-alpha (TGF-alpha), hepatocyte growth factor (HGF) and epidermal growth factor (EGF) genes expression were determined by quantitative real-time polymerase chain reaction.RESULTS: CCl4-induced acute liver failure model offers a survival benefit in baicalein-treated mice. The data indicated that the mRNA levels of IL-6 and TNF-alpha significantly increased within 12 h after CCl4 treatment in baicalein administration groups, but at 24, 48 and 72 h, the expression of IL-6 and TNF-alpha was kept at lower levels compared with the control. The expression of TGF-alpha, HGF and EGF was enhanced dramatically in baicalein administration group at 12, 24, 18 and 72 h. Furthermore, we found that baicalein significantly elevated the serum level of TNF-alpha and IL-6 at the early phase, which indicated that baicalein could facilitate the initiating events in liver regeneration.CONCLUSION: Baicalein may be a therapeutic candidate for acute liver injury. Baicalein accelerates liver regeneration by regulating TNF-alpha and IL-6 mediated pathways. (C) 2012 Baishideng. All rights reserved.