Low excitatory innervation balances high intrinsic excitability of immature dentate neurons.
Low excitatory innervation balances high intrinsic excitability of immature dentate neurons.
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DOI：
10.1038/ncomms11313
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发表时间：
2016-04-20
影响因子：
16.6
通讯作者：
Overstreet-Wadiche L
中科院分区：
文献类型：
--
作者：
Dieni CV;Panichi R;Aimone JB;Kuo CT;Wadiche JI;Overstreet-Wadiche L
齿状回中的持续神经发生产生具有高内在兴奋性和低水平抑制的未成熟神经元，其被预测比成熟神经元更广泛地响应传入活动。越来越多的证据表明，这些未成熟的神经元是产生类似背景的不同神经表征所必需的，但目前还不清楚广泛的反应神经元如何帮助区分类似的传入活动模式。在这里，我们表明，在小鼠脑切片的内嗅皮层的刺激矛盾地产生尖峰的成熟神经元的不成熟的神经元尖峰。具有高内在兴奋性的未成熟神经元由于低神经支配而不是沉默突触或低释放概率导致的兴奋驱动不足而无法尖峰。我们的研究结果表明，低突触连接阻止未成熟神经元广泛响应皮质活动，可能使兴奋的未成熟神经元，以促进稀疏和正交的齿状表示。 未成熟的齿状回神经元是高度兴奋的，并且被认为比成熟的神经元对传入活动更敏感。在这里，作者发现，由于未成熟细胞的突触连接性较低，刺激内嗅皮层矛盾地在成熟而不是未成熟的神经元中产生尖峰。
Persistent neurogenesis in the dentate gyrus produces immature neurons with high intrinsic excitability and low levels of inhibition that are predicted to be more broadly responsive to afferent activity than mature neurons. Mounting evidence suggests that these immature neurons are necessary for generating distinct neural representations of similar contexts, but it is unclear how broadly responsive neurons help distinguish between similar patterns of afferent activity. Here we show that stimulation of the entorhinal cortex in mouse brain slices paradoxically generates spiking of mature neurons in the absence of immature neuron spiking. Immature neurons with high intrinsic excitability fail to spike due to insufficient excitatory drive that results from low innervation rather than silent synapses or low release probability. Our results suggest that low synaptic connectivity prevents immature neurons from responding broadly to cortical activity, potentially enabling excitable immature neurons to contribute to sparse and orthogonal dentate representations. Immature dentate gyrus neurons are highly excitable and are thought to be more responsive to afferent activity than mature neurons. Here, the authors find stimulation of the entorhinal cortex paradoxically generates spiking in mature rather than immature neurons due to low synaptic connectivity of immature cells.