Seed size is determined by the combinations of the genes controlling different seed characteristics in rice
Seed size is determined by the combinations of the genes controlling different seed characteristics in rice
复制标题
种子大小是由控制水稻不同种子特性的基因组合决定的
DOI：
10.1007/s00122-011-1657-x
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发表时间：
2011-11-01
影响因子：
5.4
通讯作者：
Tao, Yuezhi
中科院分区：
文献类型：
--
作者：
Yan, Song;Zou, Guihua;Tao, Yuezhi
水稻籽粒大小是决定产量潜力的重要农艺性状，目前已在水稻中克隆到4个与籽粒大小相关的基因(GS3、GW2、qSW5/GW5和GIF1)。然而，这四个基因之间的关系尚不清楚，这在一定程度上阻碍了基因聚合育种的进程。为了揭示上述四个基因之间的相互关系，我们利用GS3-RNAi、GW2-RNAi系和qSW5的CSSl在转录水平上进行了基因表达分析。结果表明，qSW5和GW2对GS3的表达有正向调节作用。同时，抑制GW2转录可下调qSW5的表达。此外，GIF1的表达受qSW5的正调控，而受GW2和GS3的负调控。此外，基于180个水稻品种的自然群体，对qSW5和GS3的等位基因效应进行了详细的表征。结果表明，两个基因之间存在互作，其中影响粒长的qSW5被GS3等位基因所掩盖，影响粒宽的GS3被qSW5等位基因所掩盖。这些发现为水稻种子大小发育的分子机制提供了更多的见解，并可能对提高水稻产量有用。
Rice seed size is an important agronomic trait in determining the yield potential, and four seed size related genes (GS3,GW2,qSW5/GW5andGIF1) have been cloned in rice so far. However, the relationship among these four genes is still unclear, which will impede the process of gene pyramiding breeding program to some extent. To shade light on the relationship of above four genes, gene expression analysis was performed withGS3-RNAi,GW2-RNAi lines and CSSL ofqSW5at the transcriptional level. The results clearly showed thatqSW5andGW2positively regulate the expression ofGS3. Meanwhile,qSW5can be down-regulated by repression ofGW2transcription. Additionally,GIF1expression was found to be positively regulated byqSW5but negatively byGW2andGS3. Moreover, the allelic effects ofqSW5andGS3were detailedly characterized based on a natural population consisting of 180 rice cultivars. It was indicated that mutual interactions exist between the two genes, in which,qSW5affecting seed length is masked byGS3alleles, andGS3affecting seed width is masked byqSW5alleles. These findings provide more insights into the molecular mechanisms underlying seed size development in rice and are likely to be useful for improving rice grain yield.