Expression of red cell membrane proteins in erythroid precursor cells.
Expression of red cell membrane proteins in erythroid precursor cells.
复制标题
红细胞膜蛋白在红系前体细胞中的表达。
DOI：
10.1002/jss.400130213
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Furthmayr,H
中科院分区：
文献类型：
--
作者：
Yurchenco,PD;Furthmayr,H
人血型糖蛋白A和血影蛋白的特异性抗体被用来研究这些膜蛋白在正常和病理人骨髓中的表达。在免疫荧光实验中，血影蛋白和血型糖蛋白A存在于正常骨髓中50-60%的有核细胞中。这两种蛋白质在红细胞分化的所有阶段都有表达，并且至少可以追溯到最早的形态学上可识别的有核红细胞前体，原成红细胞;这两种蛋白质对红细胞系列的细胞具有特异性，并且未发现在淋巴细胞、粒细胞或血小板中表达。这些结论来自于对在干细胞或原成红细胞水平上红细胞生成暂时性阻滞的患者骨髓的研究。这些人的骨髓要么缺乏血型糖蛋白A和血影蛋白的所有有核细胞，要么只含有原变形细胞。通过多次注射N-乙酰-苯肼使小鼠严重贫血，在小鼠脾细胞中获得了类似的结果。从小鼠红细胞膜分离的唾液酸糖蛋白的抗体染色贫血动物脾脏中70-80%的所有细胞，而在正常动物的脾脏中仅观察到1-2%的此类细胞。Spectrin和血型糖蛋白A可以代谢标记，并使用特异性抗体分离。人肿瘤细胞系K562表达这两种膜蛋白，但迄今为止用各种试剂进行的诱导实验未能改变其表达。
Specific antibodies to human glycophorin A and spectrin were used to study the expression of these membrane proteins in normal and pathologic human bone marrow. In immunofluorescence experiments spectrin and glycophorin A are found in 50–60% of the nucleated cells in normal bone marrow. These two proteins are expressed at all stages of red cell differentiation and can be traced at least to the earliest morphologically recognizable nucleated red cell precursor, the proerythroblast; the two proteins are specific for cells of the red cell series and are not found to be expressed in lymphocytic, granulocytic cells or platelets. These conclusions were drawn from studies on bone marrow in patients with a temporary block in erythropoiesis at the level of stem cells or of the pronormoblast. Bone marrow from these individuals either lacked all nucleated cells stainable for glycophorin A and spectrin or contained only pronormoblasts. Similar findings were obtained on spleen cells from mice which were made severely anemic by multiple injections with N‐acetyl‐phenylhydrazine. Antibodies to a sialoglycoprotein isolated from mouse red cell membranes stain 70–80% of all cells in the spleen of anemic animals, while only 1–2% of such cells are seen in the spleen of normal animals. Spectrin and glycophorin A could be labeled metabolically and isolated using specific antibodies. The human tumor cell line K562 expresses both membrane proteins, but induction experiments with various agents thus far have failed to change their expression.