Flow cytometry analysis of drug transport mechanisms in Haemonchus contortus susceptible or resistant to anthelmintics
Flow cytometry analysis of drug transport mechanisms in Haemonchus contortus susceptible or resistant to anthelmintics
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DOI：
10.1007/s004360050519
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发表时间：
1999-02-01
影响因子：
2
通讯作者：
Aycardi, J
中科院分区：
文献类型：
--
作者：
Kerboeuf, D;Chambrier, P;Aycardi, J
使用流式细胞术方法检查了膜药物转运机制在抗蠕虫药中的作用。该方法改编自为研究肿瘤细胞中类似机制而开发的测定。P-糖蛋白转运探针罗丹明123与逆转剂维拉帕米联用，在抗捻转血矛线虫卵中产生的绿色荧光显著高于敏感卵。同样，用于检测细胞多药耐药性的新型荧光探针verapamilbodipy与耐药卵的结合程度显著更高。这些结果证实了使用驱虫剂和维拉帕米的生物药物测定所获得的结果，并为线虫多药耐药性的功能研究提供了一种定量和有效的方法。
The role of membrane drug-transport mechanisms in resistance to anthelmintics was examined using a flow cytometry method. This method was adapted from assays developed for the study of similar mechanisms in tumor cells. Rhodamine 123, a P-glycoprotein transport probe, associated with the reversal agent verapamil gave a significantly higher level of green fluorescence in Haemonchus contortus-resistant eggs as compared with that of susceptible eggs. In the same way, verapamilbodipy, a new fluorescent probe for the detection of multidrug resistance in cells, showed a significantly higher degree of binding to resistant eggs. The results confirm those obtained with biological drug assays using both anthelmintics and verapamil and provide a quantitative and effective methodology for the functional study of multidrug resistance in nematodes.