A dominant role for CD8+-T-lymphocyte selection in simian immunodeficiency virus sequence variation
A dominant role for CD8+-T-lymphocyte selection in simian immunodeficiency virus sequence variation
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DOI：
10.1128/jvi.78.24.14012-14022.2004
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发表时间：
2004-12-01
影响因子：
5.4
通讯作者：
Watkins, DI
中科院分区：
文献类型：
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作者：
O'Connor, DH;McDermott, AB;Watkins, DI
CD8(+)T淋巴细胞(CD8-TL)在人类免疫缺陷病毒和猴免疫缺陷病毒(SIV)感染中都选择病毒逃逸变异体。CD8-TL病毒逃逸的频率以及逃逸对整体病毒多样性的贡献尚未得到评估。我们通过对安乐死时35只SIVmac239感染动物的病毒基因组测序，量化了SIV感染中CD8-TL的选择。在这里，我们证明了对编码46个已知CD8-TL表位的序列的正选择与对env可变环观察到的正选择相当。我们还发现，病毒包膜外60%的病毒变异发生在公认的CD8-TL表位内。因此，我们得出结论，CD8-TL的选择是SIV多样性的主要原因。
CD8(+) T lymphocytes (CD8-TL) select viral escape variants in both human immunodeficiency virus and simian immunodeficiency virus (SIV) infections. The frequency of CD8-TL viral escape as well as the contribution of escape to overall virus diversification has not been assessed. We quantified CD8-TL selection in SIV infections by sequencing viral genomes from 35 SIVmac239-infected animals at the time of euthanasia. Here we show that positive selection for sequences encoding 46 known CD8-TL epitopes is comparable to the positive selection observed for the variable loops of env. We also found that >60% of viral variation outside of the viral envelope occurs within recognized CD8-TL epitopes. Therefore, we conclude that CD8-TL selection is the dominant cause of SIV diversification outside of the envelope.