Quantitative Temporal Viromics
Quantitative Temporal Viromics
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DOI：
10.1146/annurev-virology-091919-104458
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发表时间：
2021-01-01
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影响因子：
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通讯作者：
Weekes, Michael P.
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文献类型：
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作者：
Fletcher-Etherington, Alice;Weekes, Michael P.
许多宿主细胞和病毒蛋白质的丰度、定位、修饰和蛋白质-蛋白质相互作用可以在任何病毒感染的整个过程中动态变化。研究这些变化对于全面了解病毒如何复制和引起疾病以及开发抗病毒疗法和疫苗至关重要。在此之前，我们开发了一种基于质谱的技术，称为定量时间病毒组学（QTV），它采用同量异序串联质量标签（TMT），允许通过整个感染时间过程的宿主和病毒蛋白质组的精确比较定量。在这篇综述中，我们讨论了QTV的效用和应用，例证了许多研究，因为使用蛋白质组学与各种定量技术来研究病毒感染的时间。
The abundance, localization, modifications, and protein-protein interactions of many host cell and virus proteins can change dynamically throughout the course of any viral infection. Studying these changes is critical for a comprehensive understanding of how viruses replicate and cause disease, as well as for the development of antiviral therapeutics and vaccines. Previously, we developed a mass spectrometry-based technique called quantitative temporal viromics (QTV), which employs isobaric tandem mass tags (TMTs) to allow precise comparative quantification of host and virus proteomes through a whole time course of infection. In this review, we discuss the utility and applications of QTV, exemplified by numerous studies that have since used proteomics with a variety of quantitative techniques to study virus infection through time.