Development of serological assays for Thottapalayam virus, an insectivore-borne Hantavirus
Development of serological assays for Thottapalayam virus, an insectivore-borne Hantavirus
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DOI：
10.1128/cvi.00347-06
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发表时间：
2007-02-01
影响因子：
--
通讯作者：
Arikawa, Jiro
中科院分区：
文献类型：
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作者：
Okumura, Megumi;Yoshimatsu, Kumiko;Arikawa, Jiro
Thottapalayam 病毒 (TPMV) 是布尼亚病毒科汉坦病毒属的一员，是从 1964 年在印度南部捕获的食虫动物 Suncus murinus（麝鼩）中分离出来的。虽然 TPMV 的分离早于汉坦病毒原型的发现，但人们对其遗传学和生物学知之甚少。迄今为止，初步证据表明，TPMV 在抗原性和遗传性方面与所有已知的啮齿动物传播的汉坦病毒有显着差异。然而，由于尚未进行详细的动物流行病学研究，目前尚不清楚 TPMV 是否由食虫动物宿主自然携带，或者 TPMV 是否代表其天然啮齿动物宿主的“溢出”。此外，TPMV 在多大程度上引起人类感染和/或疾病尚不清楚。为了解决这些问题，我们首先使用抗汉坦病毒和首尔病毒的单克隆抗体以及抗普马拉病毒和 TPMV 的多克隆免疫血清研究了 TPMV 的抗原谱。有了这些新发现的信息，我们开发了一种酶联免疫吸附测定系统，用于诊断鼩鼱和人类的 TPMV 感染，该系统使用重组 TPMV N 抗原，该抗原经操作后具有 E5/G6 表位，可被单克隆抗体克隆 E5/G6 捕获。通过这种检测，我们在泰国一名不明原因高烧患者和印度尼西亚捕获的两只鼩鼱的血清中发现了抗 TPMV 抗体。通过间接免疫荧光抗体试验、蛋白质印迹分析和焦点减少中和试验验证血清阳性。总的来说，我们的数据表明，TPMV 在自然界中被作为其宿主的鼠鼯 (Suncus murinus) 所携带，并且能够感染人类。
Thottapalayam virus (TPMV), a member of the genus Hantavirus in the family Bunyaviridae, was isolated from an insectivore, Suncus murinus (musk shrew), captured in southern India in 1964. While the isolation of TPMV predates the discovery of the prototype Hantaan virus, little is known about its genetics and biology. To date, preliminary evidence suggests that TPMV differs significantly, both antigenically and genetically, from all known rodent-borne hantaviruses. However, since detailed epizootiological studies have not been conducted, it is unclear if TPMV is naturally harbored by an insectivore host or if TPMV represents a "spillover" from its natural rodent reservoir host. Moreover, to what extent TPMV causes infection and/or disease in humans is not known. To address these issues, we first studied the antigenic profile of TPMV using monoclonal antibodies against Hantaan and Seoul viruses and pollyclonal immune sera against Puumala virus and TPMV. Armed with this newfound information, we developed an enzyme-linked immunosorbent assay system for the diagnosis of TPMV infections in shrews and humans, using a recombinant TPMV N antigen manipulated to have an E5/G6 epitope to be captured by monoclonal antibody clone E5/G6. Using this assay, we found anti-TPMV antibodies in sera from a patient with high fever of unknown etiology in Thailand and from two shrews captured in Indonesia. Seropositivity was verified by the indirect immunofluorescence antibody test, Western blotting analysis, and focus reduction neutralization test. Collectively, our data indicate that TPMV is harbored by Suncus murinus as its host in nature and is capable of infecting humans.