Formvar membrane laid on artificial medium induces haustorium-like structure formation in powdery mildew fungi
Formvar membrane laid on artificial medium induces haustorium-like structure formation in powdery mildew fungi
复制标题
人工培养基上放置的 Formvar 膜诱导白粉病真菌形成吸器样结构
DOI：
10.1016/j.myc.2019.06.006
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发表时间：
2019
期刊：
影响因子：
1.4
通讯作者：
Takashi Yaeno
中科院分区：
文献类型：
--
作者：
Naoto Yamaoka;Eiji Tanaka;Tsubasa Ogasahara;Honoka Tani;Kappei Kobayashi;Takashi Yaeno
我们的目的是建立一个人工膜系统，以观察专性生物营养型白粉病真菌的感染过程，而不使用活的植物细胞。将小麦白粉病菌和豌豆白粉病菌的分生孢子接种在人工培养基上的福尔马林膜上。萌发的分生孢子在人工培养基中经常形成附着胞，然后穿透膜形成吸器样结构。吸器样结构形成后，还观察到次生菌丝伸长。这些结果表明，福尔马林膜在人工培养基上诱导了吸器样结构的形成，吸器样结构在次生菌丝的形成中起作用。
We aimed to develop an artificial membrane system to observe the infection process of the obligate biotrophic powdery mildew fungi without the use of living plant cells. The conidia of Blumeria graminis and Erysiphe pisi conidia were inoculated on a formvar membrane laid on an artificial medium. Germinated conidia frequently formed appressoria and then penetrated the membrane to form haustorium-like structures in the artificial medium. Secondary hyphae elongation was also observed after the formation of haustorium-like structures. These results suggested that the formvar membrane laid on artificial medium induced the formation of haustorium-like structures that have roles in the formation of secondary hyphae.