PERIODICITIES IN EFFERENT DISCHARGE OF SPLANCHNIC NERVE OF CAT
PERIODICITIES IN EFFERENT DISCHARGE OF SPLANCHNIC NERVE OF CAT
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DOI：
10.1152/ajplegacy.1970.218.4.1092
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发表时间：
1970-01-01
影响因子：
--
通讯作者：
GOOTMAN, PM
中科院分区：
文献类型：
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作者：
COHEN, MI;GOOTMAN, PM
方法在2.7- 4.3kg的猫上进行了神经肌肉阻滞（三碘没食子胺或十烃氯铵）和双侧气胸，并进行了人工通气。在乙醚诱导和初始手术后，动物保持：a）在氨基甲酸乙酯（urethan）麻醉（1.0- 1. 25 g/kg，iv）;或B）中丘去肌后未麻醉。在去大脑猫中，在50%NPO和50%OS的混合物下进行进一步手术以维持镇痛。在去大脑之前，为了保持颈动脉压力感受器放电，将颈外动脉而不是颈总动脉结扎。用100%O2对猫进行人工通气，并调节通气以给出3.8- 4.8%的潮气末CO2值，如通过快速红外CO？分析仪直肠温度保持在36.5至38 ℃之间。在许多猫中，输注了5%葡萄糖的生理盐水溶液（100 ml/kg/24 h）。左侧内脏大神经位于肋椎三角，在其进入腹腔神经节的入口处切断，并脱鞘。左膈神经位于颈部背外侧区域，尽可能远地向尾侧打结，并脱鞘。将每根神经浸没在矿物油池中，通过该矿物油池使5%COZ-95%O2混合物起泡。所有数据记录在磁带上：I）单相记录传出内脏神经活动（放大器带宽0.2- 1，000周期/组）。在一个直流记录实验中，显示了缓慢电位变化的再现的充分性，其中在直流耦合信号和RC耦合信号之间没有发现波形的差异。在某些情况下，通过高通电路（时间常数5 msec）对电位进行额外滤波（用于后续积分）。2)双向记录不同膈神经活动（带宽80- 10，000周期/组）。通过泄漏积分器电路（时间常数0.1设置）进一步处理膈电位，以获得平滑波形（10）。3)从放置在左前爪和右后爪中的一对针连续监测心电图（ECG）。4)通过斯坦森P23 Dd传感器通过股动脉导管测量动脉血压，股动脉导管的尖端位于胸主动脉中。5)气管压力，这表明人工通气的阶段，测量由斯坦森P23 BB传感器连接到侧臂在封闭的通气系统。
METHODSExperiments were performed on cats (2.7-4.3 kg) which had neuromuscular blockade(gallamine triethiodide or decamethonium chloride) and bilateral pneumothorax, and were artificially ventilated. After ether induction and initial surgery, the animals were maintained either: a) under ethyl carbamate(urethan) anesthesia(1.0-I. 25 g/kg, iv); or b) unanesthetized after midcollicular deccrebration. In decerebrate cats, further surgery was performed under a mixture of 50% NPO and 50% OS, to maintain analgesia. Before decerebration, the external rather than the common carotid arteries were tied in order to retain carotid baroreceptor discharge. The cats were artificially ventilated with 100% 02, and the ventilation was adjusted to give end-tidal CO2 values of 3.8-4.8%, as monitored by a rapid infrared CO? analyzer. Rectal temperatures were maintained between 36.5 and 38 C. In many cats 5% dextrose in physiologic saline was infused (100 ml/kg per 24 hr). The left greater splanchnic nerve was located in the costovertebral triangle, cut at its entrance into the celiac ganglion, and desheathed. The left phrenic nerve was located in the dorsolateral region of the neck, tied as far caudally as possible, and desheathed. Each nerve was irnmersed in a pool of mineral oil through which a 5% COZ-95% 02 mixture had been bubbled. All data were recorded on magnetic tape: I) Efferent splanchnic nerve activity was recorded monophasically (amplifier bandwidth 0.2-l, 000 cycles/set). The adequacy of reproduction of slow potential changes was shown in one experiment with dc recording, where no difference of waveform was found between the dc-and the RC-coupled signal. In some cases, the potentials were additionally filtered (for subsequent integration) by a high-pass circuit (time constant 5 msec). 2) kfferent phrenic nerve activity was recorded diphasically(bandwidth 80-10,000 cycles/set). The phrenic potentials were further processed through a leaky integrator circuit (time constant 0.1 set), to obtain a smoothed waveform(10). 3) The electrocardiogram (ECG) was continuously monitored from a pair of needles placed in the left forepaw and right hindpaw. 4) Arterial blood pressure was measured by a Statham P23Dd transducer through a femoral arterial catheter whose tip was located in the thoracic aorta.. 5) Tracheal pressure, which indicated the phases of artificial ventilation, was measured by a Statham P23BB transducer attached to a side arm in the closed ventilation system.