A novel phase-contrast transmission electron microscopy producing high-contrast topographic images of weak objects
A novel phase-contrast transmission electron microscopy producing high-contrast topographic images of weak objects
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DOI：
10.1023/a:1021234621466
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发表时间：
2002-01-01
影响因子：
1.8
通讯作者：
Nagayama, K
中科院分区：
文献类型：
--
作者：
Danev, R;Okawara, H;Nagayama, K
我们报告了一类新型的透射电子显微镜（TEM），差衬电子显微镜（DTEM），它显示薄样品物体的纳米结构在地形的方式。由差衬法获得的地形图发展出伪三维的阴影图，即投射物体的体积状表示，好像物体被来自一个方向的光照亮。用于DTEM的操纵电子波的特定光学装置是半圆形相位板，其看起来与Zernike相衬TEM中使用的Zernike相位板非常不同，而两者都必须放置在物镜透镜的后焦平面上。用透射电镜和动态透射电镜对肾细胞切片进行了比较。DTEM通过观察细胞内的超微结构细节证实了高对比度地形图的实验优势。
We report a novel class of transmission electron microscope (TEM), the difference-contrast electron microscope (DTEM), which displays nanostructures of thin specimen objects in a topographical manner. Topography obtained by the difference-contrast develops shadowgraphs in pseudo three-dimension, namely volume-like representation of projected objects as if things are illuminated by light from one direction. The specific optical device to manipulate electron waves for DTEM is the hemicircular phase-plate, which appears to be quite distinguishable from the Zernike phase plate utilized in Zernike phase-contrast TEM, while both have to be placed onto the back-focal plane of the objective lens. The topographic images obtained with DTEM for ultrathin sections of kidney cells were compared with those obtained with conventional TEM. DTEM confirmed the experimental advantage of high contrast topography by visualizing ultrastructural details inside the cells.