ATP detection using a label-free DNA aptamer and a cationic tetrahedralfluorene.
ATP detection using a label-free DNA aptamer and a cationic tetrahedralfluorene.
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DOI：
10.1039/b806908e
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发表时间：
2008-10
期刊：
影响因子：
--
通讯作者：
Yanyan Wang;B. Liu
中科院分区：
文献类型：
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作者：
Yanyan Wang;B. Liu
报道了一种以无标记DNA适配体为识别元件，溴化乙锭（EB）为信号报告分子的ATP检测方法。在靶结合时，ATP结合适体经历从适体双链体到适体/靶复合物的构象转换，这诱导嵌入EB发射的荧光变化。ATP与CTP、GTP或UTP之间的良好选择性已被证明，这是由于ATP适体与ATP之间的特异性识别。单独使用EB作为信号报告，ATP的检测限估计为约0.2 mM. When捕光阳离子tetrahedralfluorene被用作能量供体敏化嵌入的EB发射，10倍的检测限增加和2倍的检测选择性增加被证明。的灵敏度和选择性的tetrahedralfluorene敏化测定是可比的或优于大多数荧光ATP测定与多个标签。
A simple and sensitive method for ATP detection using a label-free DNA aptamer as the recognition element and ethidium bromide (EB) as the signal reporter is reported. The ATP-binding aptamer undergoes a conformational switch from the aptamer duplex to the aptamer/target complex upon target binding, which induces the fluorescence change of intercalated EB emission. Good selectivity between ATP and CTP, GTP or UTP has been demonstrated, which is due to the specific recognition between the ATP aptamer and ATP. Using EB alone as a signal reporter, the ATP detection limit was estimated to be approximately 0.2 mM. When a light harvesting cationic tetrahedralfluorene was used as an energy donor to sensitize the intercalated EB emission, a 10-fold increase in detection limit and a 2-fold increase in detection selectivity was demonstrated. The sensitivity and selectivity of the tetrahedralfluorene sensitized assay is comparable to or better than most fluorescent ATP assays with multiple labels.