A high-resolution gene expression atlas links dedicated meristem genes to key architectural traits
A high-resolution gene expression atlas links dedicated meristem genes to key architectural traits
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DOI：
10.1101/gr.250878.119
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发表时间：
2019-12-01
期刊：
影响因子：
7
通讯作者：
Timmermans, Marja C. P.
中科院分区：
文献类型：
--
作者：
Knauer, Steffen;Javelle, Marie;Timmermans, Marja C. P.
茎顶端分生组织（SAM）协调干细胞增殖和器官起始之间的平衡，这对胚后茎的生长至关重要。分生组织在形状和大小上表现出惊人的多样性。这种形态多样性如何与植物结构的变化和驱动它的分子电路有关尚不清楚。通过生成营养玉米茎尖的高分辨率基因表达图谱，我们在这里表明，不同的基因组管理玉米与拟南芥中干细胞的调控和身份。玉米SAM中的细胞身份反映了转录因子（TF）的组合活性，所述转录因子驱动在过多的细胞过程中起作用的基因家族内的个体成员的优先差异表达。因此，亚功能化成为细胞身份的基本特征。此外，我们表明，成年植物的特点，在很大程度上，调节基因电路作用于SAM，与自然变异调制农艺学上重要的建筑特征丰富，特别是近动态表达的SAM基因和TF，调节它们。除了玉米干细胞调控的独特机制外，我们的图谱还确定了作物改良的关键新目标。
The shoot apical meristem (SAM) orchestrates the balance between stem cell proliferation and organ initiation essential for postembryonic shoot growth. Meristems show a striking diversity in shape and size. How this morphological diversity relates to variation in plant architecture and the molecular circuitries driving it are unclear. By generating a high-resolution gene expression atlas of the vegetative maize shoot apex, we show here that distinct sets of genes govern the regulation and identity of stem cells in maize versus Arabidopsis. Cell identities in the maize SAM reflect the combinatorial activity of transcription factors (TFs) that drive the preferential, differential expression of individual members within gene families functioning in a plethora of cellular processes. Subfunctionalization thus emerges as a fundamental feature underlying cell identity. Moreover, we show that adult plant characters are, to a significant degree, regulated by gene circuitries acting in the SAM, with natural variation modulating agronomically important architectural traits enriched specifically near dynamically expressed SAM genes and the TFs that regulate them. Besides unique mechanisms of maize stem cell regulation, our atlas thus identifies key new targets for crop improvement.