Identification of 14 new phosphoproteins involved in important plant mitochondrial processes
Identification of 14 new phosphoproteins involved in important plant mitochondrial processes
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DOI：
10.1016/s0014-5793(03)00250-3
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发表时间：
2003-04-10
期刊：
影响因子：
3.5
通讯作者：
Moller, IM
中科院分区：
文献类型：
--
作者：
Bykova, NV;Egsgaard, H;Moller, IM
蛋白质磷酸化是一个非常重要的翻译后修饰，其作用在线粒体中几乎未被探索。利用双向凝胶电泳和质谱联用技术，在马铃薯块茎线粒体中鉴定出14个新的磷酸化蛋白，这些蛋白在哺乳动物和真菌线粒体中也存在。新发现的磷酸蛋白中有7种参与三羧酸循环或相关反应，4种是呼吸复合物的亚基，参与电子传递、ATP合成和蛋白质加工，2种是热休克蛋白，1种参与氧化应激防御。这些发现为线粒体过程的调控和信号整合开辟了全新的可能性。(C) 2003年欧洲生化学会联合会。Elsevier Science B.V.版权所有。
Protein phosphorylation is a very important post-translational modification the role of which is practically unexplored in mitochondria. Using two-dimensional gel electrophoresis followed by mass spectrometry, 14 new phosphoproteins are identified in potato tuber mitochondria, all household proteins also present in mammalian and fungal mitochondria. Seven of the new phosphoproteins are involved in the tricarboxylic acid cycle or associated reactions, four are subunits of respiratory complexes and involved in electron transport, ATP synthesis and protein processing, two are heat shock proteins and one is involved in defence against oxidative stress. These findings open up entirely new possibilities for the regulation and signal integration of mitochondrial processes. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.