Dimensional stability of the alveolar ridge after implantation of a bioabsorbable bone graft substitute: a radiographic and histomorphometric study in rats.
Dimensional stability of the alveolar ridge after implantation of a bioabsorbable bone graft substitute: a radiographic and histomorphometric study in rats.
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DOI：
10.1563/0-727.1
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发表时间：
2005-01-01
期刊：
影响因子：
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通讯作者：
Trantolo, Debra J
中科院分区：
文献类型：
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作者：
Hile, David D;Sonis, Stephen T;Trantolo, Debra J
本研究评估了使用生物可吸收骨修复支架的大鼠拔牙后牙槽嵴的重建。该材料由不饱和聚酯聚（丙二醇-富马酸）（PPF）制备，可以原位固化形成多孔支架。目的是使用这种材料作为独立的骨移植替代物或作为从下颌重建部位收获的自体移植物的延伸物。在大鼠残余牙槽嵴吸收模型中研究了移植物替代物的生物活性。 PPF 骨修复材料被注入缺损部位，在羟基磷灰石 (HA) 填充剂和泡腾剂存在下原位交联。这种基于 PPF 的材料在柠檬酸和碳酸氢钠的泡腾反应过程中产生二氧化碳，从而在原位固化过程中形成孔隙。 HA 的掺入可促进骨修复支架内的骨传导。在这项研究中，多孔支架的生物活性被评估为 HA 颗粒尺寸（微米尺寸与纳米尺寸颗粒）的函数。右侧上颌或下颌磨牙是从 96 只成年 Sprague-Dawley 大鼠中拔除的。使用 2 毫米圆钻在每个提取部位创建直径为 2 毫米、深度为 2 毫米、长度为 4 毫米的均匀沟槽缺陷。缺损部位（1）用含有纳米级HA的PPF骨修复材料处理，（2）用含有微米级HA的PPF材料处理，（3）用脱矿冻干同种异体骨移植物处理，或（4）不进行处理。术后2、4、7和12周处死大鼠。通过放射学结果评估残余牙槽嵴的吸收。通过组织形态测量结果来测量通过缺损部位的骨向内生长。本研究中使用的所有治疗方法下颌骨和上颌骨嵴高度均有所增加。没有临床迹象表明添加任何一种 PPF 骨修复材料会阻碍拔牙部位的硬组织或软组织愈合。尽管在统计上不显着，但与其他治疗相比，用含有纳米级 HA 的 PPF 治疗的下颌缺损愈合速度更快，这是根据牙槽嵴高度和新骨形成测量结果确定的。这些发现表明使用 PPF 骨移植替代物用于口腔颌面应用的可行性。
This study evaluated reconstruction of the alveolar ridge after molar extraction in rats with bioabsorbable bone repair scaffolds. The material was prepared from the unsaturated polyester poly(propylene glycol-co-fumaric acid) (PPF), which may be cured in situ to form a porous scaffold. The intention is to use this material either as a stand-alone bone graft substitute or as an extender to autograft harvested from mandibular reconstruction sites. The bioactivity of the graft substitute was investigated in a rat residual ridge resorption model. PPF bone repair material was injected into the defect site, where it cross-linked in situ in the presence of a hydroxyapatite (HA) filler and effervescent agents. The PPF-based material develops porosity during an in situ cure by generating carbon dioxide during the effervescent reaction of citric acid and sodium bicarbonate. The incorporation of HA promotes osteoconduction within the bone repair scaffold. In this study, bioactivity of the porous scaffold was evaluated as a function of HA particle size (micrometer-sized vs nanometer-sized particles). The maxillary or mandibular molars on the right side were extracted from 96 adult Sprague-Dawley rats. A 2-mm round bur was used to create a uniform trench defect measuring 2 mm in diameter, 2 mm in depth, and 4 mm in length at each extraction site. The defect site was (1) treated with PPF bone repair material containing nanometer-sized HA, (2) treated with PPF material containing micrometer-sized HA, (3) treated with demineralized freeze-dried bone allograft, or (4) left untreated. Rats were sacrificed at 2, 4, 7, and 12 weeks postoperative. Resorption of the residual alveolar ridge was assessed by radiographic outcomes. Bone ingrowth through the defect site was measured by histomorphometric outcomes. Mandibular and maxillary ridge heights increased for all treatments used in this study. There were no clinical indications that addition of either of the PPF bone repair materials retarded hard- or soft-tissue healing of the extraction sites. Although not statistically significant, the mandibular defects treated with PPF containing nanometer-sized HA healed at a faster rate as determined by ridge height and new bone formation measurements when compared with the other treatments. These findings suggest the feasibility of using PPF bone graft substitutes for oral-maxillofacial applications.