In vitro evaluation of electrospun nanofiber scaffolds for vascular graft application
In vitro evaluation of electrospun nanofiber scaffolds for vascular graft application
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DOI：
10.1002/jbm.a.31287
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发表时间：
2007-12-15
影响因子：
4.9
通讯作者：
Stitze, Joel
中科院分区：
文献类型：
--
作者：
Lee, Sang Jin;Yoo, James J.;Stitze, Joel
血管的大小、机械和生化特性、细胞含量和超微结构组织取决于其位置和特定功能而不同。因此，需要控制血管移植物的制造以获得血管替代物的期望特性。在这项研究中，我们已经制作了各种支架使用静电纺丝技术与胶原蛋白，弹性蛋白和几种可生物降解的聚合物的混合物。评价了生物相容性、体外尺寸稳定性和力学性能。材料以45%胶原蛋白、15%弹性蛋白和40%合成聚合物的相对重量浓度混合，以模拟天然血管中胶原蛋白和弹性蛋白的比例。所制造的支架由直径范围为477至765 nm的随机取向的纤维组成。电纺支架无毒，在体外培养环境中尺寸稳定，易于制造，并具有模拟天然血管超微结构的受控机械性能。本研究表明，合成的生物可降解聚合物的引入使定制的血管替代物的机械性能和改善顺应性匹配的血管组织工程。(c)2007 Wiley Periodicals，Inc.
Blood vessels are diverse in size, mechanical and biochemical properties, cellular content, and ultrastructural organization depending on their location and specific function. Therefore, it is required to control the fabrication of vascular grafts for obtaining desirable characteristics of blood vessel substitutes. In this study we have fabricated various scaffolds using the electrospinning technique with blends of collagen, elastin, and several biodegradable polymers. Bio-compatibility, dimensional stability in vitro and mechanical properties were evaluated. Materials were blended at a relative concentration by weight of 45% collagen, 15% elastin, and 40% synthetic polymer to mimic the ratio of collagen and elastin in native blood vessels. The fabricated scaffolds are composed of randomly oriented fibers with diameters ranging from 477 to 765 nm. The electrospun scaffolds are nontoxic, dimensionally stable in an in vitro culture environment, easily fabricated, and possess controlled mechanical properties that simulate the ultrastructure of native blood vessels. The present study suggests that the introduction of synthetic biodegradable polymers enabled tailoring of mechanical properties of vascular substitutes and improving compliance matching for vascular tissue engineering. (c) 2007 Wiley Periodicals, Inc.