Preparation, characterization and antioxidant activity of two partially N-acetylated chitotrioses
Preparation, characterization and antioxidant activity of two partially N-acetylated chitotrioses
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DOI：
10.1016/j.carbpol.2012.11.028
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发表时间：
2013-02-15
影响因子：
11.2
通讯作者：
Li, Pengcheng
中科院分区：
文献类型：
--
作者：
Li, Kecheng;Liu, Song;Li, Pengcheng
制备聚合度单一、乙酰化程度明确的壳寡糖，对鉴定具有较强生物功能的组分具有重要意义。本研究主要研究了两种部分乙酰化的壳三糖（N-乙酰化壳三糖和N，N'-二乙酰化壳三糖）的制备，包括两个步骤：(i)部分乙酰化的壳三糖和（ii）不同乙酰化的壳三糖的分离。壳聚糖经n -乙酰化，并用FT-IR和ESI-MS进行了表征。采用CM Sepharose Fast Flow从乙酰化壳聚糖混合物中分离出N-乙酰壳聚糖和N，N'-二乙酰壳聚糖。随后，研究了两种部分乙酰化壳三糖和原始壳三糖的抗氧化活性，包括清除羟基自由基和超氧自由基的活性。结果表明，三种低聚物的活性与乙酰化程度呈剂量依赖关系。乙酰化程度高的N，N'-二乙酰壳三糖抗氧化活性最高。(C) 2012 Elsevier Ltd.版权所有。
The preparation of chitooligosaccharides with single degree of polymerization and well-defined degree of acetylation is of significance to identify the components possessing strong biofunction. This study focuses on the preparation of two partially acetylated chitotrioses (N-acetylchitotriose and N,N'-diacetylchitotriose), involving a two-step process: (i) partially N-acetylation of chitotriose and (ii) separation of different acetylated chitotrioses. The chitotriose was N-acetylated and characterized with FT-IR and ESI-MS. N-acetylchitotriose and N,N'-diacetylchitotriose were separated from the acetylated chitotriose mixture by CM Sepharose Fast Flow. Subsequently, the antioxidant activities of two partially acetylated chitotrioses and original chitotriose were investigated, including hydroxyl radical and superoxide radical scavenging activity. The results showed that the activities of the three oligomers were in a dose-dependence manner and related to their degree of acetylation. The N,N'-diacetylchitotriose with high degree of acetylation exhibited the highest antioxidant activity. (C) 2012 Elsevier Ltd. All rights reserved.