Loss of CRWN Nuclear Proteins Induces Cell Death and Salicylic Acid Defense Signaling
Loss of CRWN Nuclear Proteins Induces Cell Death and Salicylic Acid Defense Signaling
复制标题
DOI：
10.1104/pp.18.01020
复制
发表时间：
2019-04-01
期刊：
影响因子：
7.4
通讯作者：
Richards, Eric J.
中科院分区：
文献类型：
--
作者：
Choi, Junsik;Strickler, Susan R.;Richards, Eric J.
动物细胞核核板层的缺陷对核结构和基因表达以及多种生理表现具有显著影响。我们报告说，在拥挤的核（CRWN），这是候选核层蛋白在拟南芥（拟南芥）的缺陷，触发转录水平和全植物表型，包括矮化和自发细胞死亡病变的广泛变化。这些表型部分是由通过水杨酸途径异位诱导植物防御反应引起的。CRWN蛋白质的丢失诱导水杨酸生物合成基因ISOCHORISMATE SYNTHASE 1的表达，这导致crwn 1 crwn 2和crwn 1 crwn 4突变体的自发防御反应，这是四种CRWN旁系同源物中的两种缺陷。异位防御反应的症状，包括发病标志基因表达和细胞死亡，在老年crwn双突变体增加。这些年龄依赖性效应被假定为反映了随着时间的推移核功能障碍或损伤的增加，这一现象让人联想到在动物细胞核和一些人类核纤层蛋白病患者中观察到的衰老效应。
Defects in the nuclear lamina of animal cell nuclei have dramatic effects on nuclear structure and gene expression as well as diverse physiological manifestations. We report that deficiencies in CROWDED NUCLEI (CRWN), which are candidate nuclear lamina proteins in Arabidopsis (Arabidopsis thaliana), trigger widespread changes in transcript levels and whole-plant phenotypes, including dwarfing and spontaneous cell death lesions. These phenotypes are caused in part by ectopic induction of plant defense responses via the salicylic acid pathway. Loss of CRWN proteins induces the expression of the salicylic acid biosynthetic gene ISOCHORISMATE SYNTHASE1, which leads to spontaneous defense responses in crwn1 crwn2 and crwn1 crwn4 mutants, which are deficient in two of the four CRWN paralogs. The symptoms of ectopic defense response, including pathogenesis marker gene expression and cell death, increase in older crwn double mutants. These age-dependent effects are postulated to reflect an increase in nuclear dysfunction or damage over time, a phenomenon reminiscent of aging effects seen in animal nuclei and in some human laminopathy patients.