Bacillus horti sp. nov., a new gram-negative alkaliphilic bacillus.
Bacillus horti sp. nov., a new gram-negative alkaliphilic bacillus.
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园艺芽孢杆菌
DOI：
10.1099/00207713-48-2-565
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
H. Shinano
中科院分区：
文献类型：
--
作者：
I. Yumoto;Koji Yamazaki;T. Sawabe;K. Nakano;K. Kawasaki;Y. Ezura;H. Shinano
从日本北海道厚真市的土壤中分离出新的革兰氏阴性嗜碱性菌株。这些分离株是严格需氧的杆状体，产生位于末端的椭圆形孢子。分离株的化学分类学特征包括细胞壁中存在内消旋二氨基庚二酸以及 DNA G + C 含量为 40.2-40.9 mol%。主要的类异戊二烯醌是 menaquinone-7，细胞脂肪酸谱由大量 15-C 支链酸、iso-C15:0 和 anteiso-C15:0 组成。 pH 8-10 时的生长速率高于 pH 7 时的生长速率。对 14 株嗜碱性芽孢杆菌菌株的 16S rDNA 进行比较序列分析表明，分离的菌株与嗜碱性芽孢杆菌属物种的 3 个已定义的 rRNA 组具有等距关系。根据形态和生理特征，以及通过 16S rDNA 分析和 DNA-DNA 相关性数据确定的系统发育位置，得出的结论是，这些分离株应被指定为一个新种，建议将其命名为 Bacillus horti。模式菌株是 K13T（= JCM 9943T）。
Novel Gram-negative alkaliphilic strains were isolated from soil obtained from Atsuma, Hokkaido, Japan. The isolates were strictly aerobic rods that produced subterminally located ellipsoidal spores. Chemotaxonomic characteristics of the isolates included the presence of meso-diaminopimelic acid in the cell wall and a DNA G + C content of 40.2-40.9 mol%. The major isoprenoid quinone was menaquinone-7 and the cellular fatty acid profile consisted of a significant amount of 15-C branched-chain acids, iso-C15:0 and anteiso-C15:0. The growth rate was higher at pH 8-10 than at pH 7. Comparative sequence analysis of 16S rDNA of 14 alkaliphilic Bacillus strains indicates that the isolated strain has an equidistant relationship to three already defined rRNA groups of alkaliphilic Bacillus species. Based on the morphological and physiological characteristics, as well as phylogenetic position as determined by 16S rDNA analysis and DNA-DNA relatedness data, it is concluded that these isolates should be designated as a new species, for which the name Bacillus horti is proposed. The type strain is K13T (= JCM 9943T).