DL-3-n-Butylphthalide, an Anti-Oxidant Agent, Prevents Neurological Deficits and Cerebral Injury Following Stroke per Functional Analysis, Magnetic Resonance Imaging and Histological Assessment
DL-3-n-Butylphthalide, an Anti-Oxidant Agent, Prevents Neurological Deficits and Cerebral Injury Following Stroke per Functional Analysis, Magnetic Resonance Imaging and Histological Assessment
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DOI：
10.2174/156720212801618956
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发表时间：
2012-08-01
影响因子：
2.1
通讯作者：
Yew, D. T.
中科院分区：
文献类型：
--
作者：
Zhang, Lihong;Yu, Wan-hua Amy;Yew, D. T.
DL-3-正丁基苯酞（NBP）是以L-3-正丁基苯酞为原料，从芹菜种子中分离得到的人工合成化合物。本研究的目的是评估在自发性高血压大鼠（SHR）和血压正常的Wistar京都大鼠（WKY）缺血性中风发作之前和之后给予NBP的结果。采用大脑中动脉阻塞（MCAO）法建立SHR和WKY大鼠脑卒中模型。对于预处理，在MCAO之前每天给予SHR和WKY NBP两个月。对于治疗后，在MCAO后连续7天每天给予NBP。手术后7天，测试大鼠是否存在神经缺陷。磁共振成像（MRI）和氯化三苯基四氮唑（TTC）染色计算梗死体积。在显微镜下观察缺血半暗带区大脑皮质和纹状体的病理变化。在SHR，NBP治疗前和治疗后显着降低神经功能缺损评分，减少梗死体积，并最大限度地减少病理变化的半影区相比，石油溶剂处理的控制。在WKY中，仅在治疗后组中观察到这些有益效果。NBP后处理的有益效果在WKY中大于SHR。结果表明，NBP对SHR缺血性脑卒中有预防和治疗作用，而对WKY仅有治疗作用。
DL-3-n-Butylphthalide (NBP) is a synthetic compound based on L-3-n-Butylphthalide which was isolated from seeds of Apium graveolens. The present study aims at evaluating the outcome of NBP given prior to and after the onset of ischemic stroke in spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto rats (WKY). Stroke was induced by the middle cerebral artery occlusion (MCAO) in SHR and WKY. For pre-treatment, NBP was administered to SHR and WKY daily for two months prior to MCAO. For post-treatment, NBP was given daily for seven consecutive days after MCAO. Seven days post-surgery, rats were tested for the presence of neurological deficits. Magnetic resonance imaging (MRI) and 2,3,5-triphenyltetrazolium chloride (TTC) staining were employed to calculate the infarct volume. The cerebral cortex and corpus striatum in the ischemic penumbra area were examined microscopically for pathological changes. In SHR, NBP pre- and post-treatment significantly lowered neurological deficit scores, reduced infarct volume, and minimized pathological changes in the penumbra area when compared to oil-vehicle treated controls. In WKY, these beneficial effects were observed only in the post-treatment group. The beneficial effects of NBP post-treatment were greater in WKY than in SHR. Results indicated that NBP could exert both preventive and therapeutic effects on ischemic stroke in SHR, but only exerted therapeutic effect in WKY.