Flow cytometric immunophenotyping of cerebrospinal fluid.
Flow cytometric immunophenotyping of cerebrospinal fluid.
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脑脊液的流式细胞术免疫表型分析。
DOI：
10.1002/0471142956.cy0625s45
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发表时间：
2008
影响因子：
--
通讯作者：
Wilson,Wyndham
中科院分区：
文献类型：
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作者：
Kraan,Jaco;Gratama,JanW;Haioun,Corinne;Orfao,Alberto;Plonquet,Anne;Porwit,Anna;Quijano,Sandra;Stetler-Stevenson,Maryalice;Subira,Dolores;Wilson,Wyndham
软脑膜病是发生在B和T细胞淋巴瘤以及淋巴和骨髓来源的急性白血病患者中的重要不良并发症。最近的报告表明，多参数流式细胞术免疫表型评估的脊髓液样本可以提高检测效率的CNS参与，由于其高特异性和更高的灵敏度。然而，脊髓液样品通常是少细胞的，细胞活力迅速下降。因此，脊髓液的染色需要专门的样品储存/运输、染色和制备方案。本单元的基本方案概述了一种共识多参数（3至8色）流式细胞术免疫表型方案，用于评价肿瘤细胞对脑脊液（CSF）样本CNS的累及。还提供了描述同时评估表面和细胞质抗原的支持方案。最后，在替代方案中，我们描述了一种通过向测定中添加计数珠来计算正常和病理细胞亚群的绝对数量的方法。议定书Cytom 45：6.25.1 - 6.25.16。© 2008年由约翰威利父子公司。
Leptomeningeal disease is an important adverse complication occurring in patients with B and T cell lymphomas and acute leukemias of lymphoid and myeloid origin. Recent reports suggest that multiparameter flow cytometry immunophenotypic assessment of spinal fluid samples could improve the efficiency of detection of CNS involvement, due to its high specificity and greater sensitivity. However, spinal fluid samples are frequently paucicellular with a rapidly decreasing cell viability. Staining of spinal fluid therefore requires dedicated sample storage/transport, staining, and preparation protocols. The Basic Protocol in this unit outlines a consensus multiparameter (3‐ to 8‐color) flow cytometry immunophenotypic protocol for the evaluation of CNS involvement of cerebrospinal fluid (CSF) samples by neoplastic cells. A Support Protocol describing the simultaneous assessment of surface and cytoplasmic antigens is also provided. Finally, in the Alternate Protocol, we describe a method to calculate absolute numbers of both normal and pathological cell subpopulations by adding counting beads to the assay.Curr. Protocol. Cytom. 45:6.25.1‐6.25.16. © 2008 by John Wiley & Sons, Inc.