Helicobacter species in the human colon
Helicobacter species in the human colon
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DOI：
10.1111/j.1463-1318.2008.01672.x
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发表时间：
2010-01-01
影响因子：
3.4
通讯作者：
Frizelle, F. A.
中科院分区：
文献类型：
--
作者：
Keenan, J. I.;Beaugie, C. R.;Frizelle, F. A.
目的了解幽门螺杆菌在结肠中的流行程度、频率和定植模式。方法首次结肠镜检查非特异性胃肠道紊乱的患者在结肠镜检查期间从多达四个部位进行结肠活检，并使用群体特异性PCR检查幽门杆菌家族的证据。同时采集血清，检测IgG对幽门螺杆菌的反应性。结果100例患者行结肠镜检查，其中35例发现全结肠有幽门螺杆菌的DNA证据，22例有幽门螺杆菌。15例患者对幽门螺杆菌有明显的血清IgG反应，这并不总是与结肠活检中幽门螺杆菌DNA的分子证据相关，反之亦然。在幽门螺杆菌感染患者中未发现与结肠疾病的特异性关联。结论：我们在大量结肠镜检查患者中发现幽门螺杆菌感染的证据，但这些细菌的存在与结肠疾病之间没有特定的关联。我们发现分子技术和血清学技术检测幽门螺杆菌种类之间存在差异，这表明未来的研究不应仅依靠血清学来检测人类结肠中的这些细菌。
ObjectiveTo determine the prevalence, frequency and colonization patterns of Helicobacter species throughout the colon.MethodPatients having initial colonoscopy for nonspecific gastrointestinal disturbance had colonic biopsies taken from up to four sites during colonoscopy and examined for evidence of the Helicobacteraceae family using a group-specific PCR. Serum was also collected and examined for IgG reactivity to Helicobacter pylori.Results100 patients had colonoscopy of whom 35 were found to have DNA evidence of Helicobacter species throughout the colon, with 22 having H. pylori. Fifteen patients had a demonstrable serum IgG response to H. pylori that was not always associated with molecular evidence of H. pylori DNA in colon biopsies and vice versa. No specific association with colon disease was found in patients with H. pylori infection.ConclusionWe found evidence of Helicobacter infection in a significant number of patients presenting for colonoscopy but no specific association between the presence of these bacteria and colon disease. Our finding of disparity between molecular and serological techniques to detect Helicobacter species suggests that future studies should not rely on serology alone to detect these bacteria in the human colon.