Analyses of long non-coding RNA and mRNA profiling using RNA sequencing in calcium oxalate monohydrate-stimulated renal tubular epithelial cells
Analyses of long non-coding RNA and mRNA profiling using RNA sequencing in calcium oxalate monohydrate-stimulated renal tubular epithelial cells
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DOI：
10.1007/s00240-018-1065-7
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发表时间：
2019-06-01
期刊：
影响因子：
3.1
通讯作者：
Liang, Hui
中科院分区：
文献类型：
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作者：
Wang, Zhu;Zhang, Jian-wen;Liang, Hui
研究一水草酸钙附着的HK-2细胞中lncRNA和mRNA的表达谱，并探讨关键lncRNA表达水平与肾损伤之间的关系。 HK-2细胞用草酸钙晶体悬浮液处理。通过MTT法测定草酸钙晶体对HK-2细胞生长的影响。提取总RNA，通过高通量转录酶测序平台HiSeq 2500分析lncRNA和mRNA表达谱。通过实时PCR验证鉴定的lncRNA和mRNA表达谱，并通过Gene Ontology数据库和KEGG信号通路分析分析其潜在功能。草酸钙晶体在几分钟内粘附在HK-2细胞表面，表现出明显的细胞毒性。 RNA seq结果显示，草酸钙晶体处理的HK-2细胞中有25个差异表达的lncRNA，其中9个上调，16个下调。实时荧光定量PCR验证了差异具有统计学意义（P
To study the expression profiles of lncRNA and mRNA in the calcium oxalate monohydrate-attached HK-2 cells, and investigate the association between critical lncRNA expression level and renal injury. The HK-2 cells were treated with crystal suspension of calcium oxalate. The effects of calcium oxalate crystals on the growth of HK-2 cells were determined by MTT assay. Total RNA was extracted and the lncRNA and mRNA expression profiles were analyzed by high-throughput transcriptase sequencing platform HiSeq 2500. The profile of identified lncRNAs and mRNAs were verified by real-time PCR and their potential function was analyzed by Gene Ontology database and KEGG signal pathway analysis. Calcium oxalate crystals adhered to the surface of HK-2 cells in few minutes and showed obvious cytotoxicity. RNA seq results showed that there were 25 differentially expressed lncRNAs in HK-2 cells treated with calcium oxalate crystals, of which 9 were up-regulated and 16 were down-regulated. The difference was verified by real-time PCR which showed statistically significant (P