Morphometry and Lymph Dynamics of Swine Thoracic Duct
Morphometry and Lymph Dynamics of Swine Thoracic Duct
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DOI：
10.1089/lrb.2019.0069
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发表时间：
2020-03-23
影响因子：
1.4
通讯作者：
Kassab，Ghassan S.
中科院分区：
文献类型：
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作者：
Lu，Xiao;Wang，Mengjun;Kassab，Ghassan S.
背景：本研究的目的是表征胸导管（TD）的形态学和血流动力学。方法和结果：在麻醉猪（n= 9）的乳糜池（CC）的淋巴流量和压力梯度的淋巴静脉交界处进行了测定。在对动物实施安乐死后，收获TD进行组织形态学分析，其中用9%明胶灌注三个样本，以获得TD瓣膜在打开和关闭配置下的形态学。进行了光谱分析。在动物被安乐死以进行铸型（n= 3）后，进入CC的传入淋巴管并插管，以获得形态学数据。体内流率为0.7 ± 0.49mL/min。频谱分析（快速傅立叶变换）显示TD和JVP的相关系数分别为0.858 ± 0.063和0.586 ± 0.112（p< 0.05）。沿TD的平均压差为8.1 mmHg（沿着TD）。TD的长度为35.6 ± 2.2 cm。CC最大宽度为11.4 ~ 15 mm。TD的直径在2至4.3 mm之间不规则变化。TD瓣叶的几何形状确定为面积为1.99 ± 0.53 mm 2，瓣叶长度为3.26 ± 0.86 mm，包深为0.66 ± 0.19 mm，壁长为5.46 ± 2.16 mm。TD介质厚度为0.7 ± 3 μm。瓣膜的数量范围从9到13在TD的全长。结论：在猪TD的相对恒定的压力梯度驱动从CC到颈静脉的淋巴流。TD是一种薄壁血管，具有防止淋巴液回流的瓣膜。这项形态测量和淋巴动力学的研究对于介入医生了解TD的解剖学和生理学以设计新的诊断、介入手术和器械非常重要。
Background:The goal of this study was to characterize the thoracic duct (TD) both morphologically and hemodynamically.Methods and Results:The lymphatic flow and pressure gradient from the cisterna chyli (CC) to the lymphovenous junction were measured in anesthetized swine (n= 9). After the animals were euthanized, the TD were harvested for histomorphometric analyses in which three samples were perfused with 9% gelatin to obtain the morphometry of the TD valve in both the open and closed configuration. Spectral analyses were performed. An afferent lymphatic vessel of the CC was accessed and cannulated after the animal was euthanized for casting (n= 3) to obtain morphometric data. Thein vivoflow rate was 0.7 ± 0.49 mL/minute. Spectral analysis (Fast Fourier Transformation) showed correlation coefficients of 0.858 ± 0.063 and 0.586 ± 0.112 (p< 0.05) for the TD and JVPs, respectively. The average pressure gradient was 8.1 mmHg along the TD. The length of the TD was 35.6 ± 2.2 cm. The maximal width of the CC ranged from 11.4 to 15 mm. The diameter of the TD varied irregularly from 2 to 4.3 mm. The geometry of the TD leaflets was determined to have an area of 1.99 ± 0.53 mm2, a leaflet length of 3.26 ± 0.86 mm, a packet depth of 0.66 ± 0.19 mm, and a wall length of 5.46 ± 2.16 mm. The TD media thickness was ∼7 ± 3 μm. The number of valves ranged from 9 to 13 in the full length of the TD.Conclusions:A relatively constant pressure gradient in the swine TD drives lymph flow from the CC to the jugular vein. The TD is a thin-walled vessel with valves that prevent reflux of lymph flow. This study of morphometric and lymphatic dynamics is important for interventionalists to understand the anatomy and physiology of the TD to design new diagnostic, interventional procedures, and devices.