The Antigen-Antibody Reaction in Immunohistochemistry
The Antigen-Antibody Reaction in Immunohistochemistry
复制标题
免疫组织化学中的抗原抗体反应
DOI：
10.1177/002215540305100101
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发表时间：
2003
影响因子：
3.2
通讯作者：
C. Montero
中科院分区：
文献类型：
--
作者：
C. Montero
本文按以下顺序讨论了免疫组化实践中主要关心的问题：（a）与体外反应相反，在固定组织中Ag-Ab反应的机理;（B）固定的化学及其对免疫组化反应最终结果的影响;（c）在甲醛固定的组织中用于抗原修复的各种程序;以及（d）考虑热诱导抗原修复的可能机制。建议进一步的工作，试图澄清的机制参与的Ag-Ab反应在免疫组织化学诉诸现有的组织化学方法的蛋白质侧基的演示，连同一些例子已经出版。
The problems of major concern in immunohistochemical practice are discussed in the following order: (a) the mechanism of the Ag-Ab reaction in fixed tissue as opposed to the in vitro reaction; (b) the chemistry of fixation and its influence on the final result of the immunohistochemical reaction; (c) the various procedures used for antigen retrieval in formaldehyde-fixed tissue; and (d) the consideration of the possible mechanism underlying heat-induced antigen retrieval. Suggestions for further work to attempt a clarification of the mechanism involved in the Ag-Ab reaction in immunohistochemistry resorting to existing histochemical methods for the demonstration of protein side groups are presented, together with some examples already published.