PROPERTIES OF GAMMA HEMOLYSIN OF STAPHYLOCOCCUS-AUREUS SMITH-5R
PROPERTIES OF GAMMA HEMOLYSIN OF STAPHYLOCOCCUS-AUREUS SMITH-5R
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DOI：
10.1099/00221287-92-1-11
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发表时间：
1976-01-01
期刊：
影响因子：
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通讯作者：
WISEMAN, GM
中科院分区：
文献类型：
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作者：
FACKRELL, HB;WISEMAN, GM
纯化的.gamma。金黄色葡萄球菌溶血素的特征在于与α、β的关系。和.delta。溶血素。 γ的沉降系数[S20,w]。溶素为2.65，略高于新鲜纯化的α溶素的S20,w值1.4和β溶素的S20,w值1.8。赖氨酸。 .gamma 的MW。通过凝胶过滤测定溶素为45,000道尔顿。 γ 的 pI [等电点]。溶素为6.0，而α、β为6.0。和.delta。溶素范围为 8.5-9.6。 γ的氨基酸分析。溶素的特点是蛋氨酸和组氨酸含量低。甲硫氨酸是 N 末端，这表明所有氨基酸可能都参与 N 末端基团。 .gamma。溶素在免疫学上与α、β不同。和.delta。通过定量沉淀素测试测定溶素；在 Ouchterlony 琼脂凝胶扩散试验中，观察到单线沉淀，这表明 4 种溶血素之间没有交叉反应的证据。 .gamma.、.beta.和.delta。当以0-100μg的增量剂量注射时，溶素对小鼠没有作用。 .alpha。溶素杀死小鼠，LD50剂量为0.68.+-。 0.12μg，或27-34μg/kg小鼠组织。 .gamma。当心内注射50μg的溶素时，溶素对豚鼠是致命的。 .gamma。溶素还可以裂解人类白细胞并破坏 C-6（人类淋巴母细胞）细胞。一些N和P从用γ处理的人红细胞膜中释放出来。与未处理的细胞相比，溶素的释放率在 3 小时内呈线性。 .gamma。溶素对从红细胞中提取的磷脂或无脂膜蛋白没有可检测到的影响。当将红细胞膜添加到溶素-红细胞悬浮液中时，溶血反应被红细胞膜抑制；人红细胞磷脂竞争性抑制溶血。 EDTA 还可抑制溶血，并可通过透析恢复活性。溶血反应需要Na+。
Purified .gamma. hemolysin of S. aureus was characterized in relation to the .alpha., .beta. and .delta. hemolysins. The sedimentation coefficient [S20,w] of the .gamma. lysin was 2.65, somewhat higher than the S20,w values of 1.4 for freshly purified alpha lysin and 1.8 for the .beta. lysin. The MW of .gamma. lysin determined by gel filtration was 45,000 daltons. The pI [isoelectric point] of .gamma. lysin was 6.0, while that of the .alpha., .beta. and .delta. lysins ranged from 8.5-9.6. The amino acid analysis of .gamma. lysin was characterized by low levels of methionine and histidine. Methionine was the N-terminus, which suggested that all of the amino acid might be involved in the N-terminal group. The .gamma. lysin was immunologically distinct from the .alpha., .beta. and .delta. lysins by quantitative precipitin tests; in Ouchterlony agar gel diffusion tests, single lines of precipitation were observed which showed no evidence of cross-reactions among the 4 hemolysins. The .gamma., .beta. and .delta. lysins had no effect in mice when injected at increasing doses ranging from 0-100 .mu.g. The .alpha. lysin killed mice, the LD50 dose being 0.68 .+-. 0.12 .mu.g, or 27-34 .mu.g/kg mouse tissue. The .gamma. lysin was lethal for guinea pigs when 50 .mu.g quantities were injected intracardially. The .gamma. lysin also lysed human leukocytes and destroyed C-6 (human lymphoblast) cells. Some N and P was released from human erythrocyte membranes treated with .gamma. lysin, when compared to untreated cells, and the rate of this release was linear over a 3 h period. The .gamma. lysin had no detectable effect on phospholipids extracted from erythrocytes or on lipid-free membrane protein. The hemolytic reaction was inhibited by erythrocyte membranes when these were added to lysin-red cell suspensions; human red cell phospholipids competitively inhibited hemolysis. Hemolysis was also inhibited by EDTA and activity could be restored by dialysis. The hemolytic reaction required Na+.