SECRETION OF CELLULASE AND BETA-GLUCOSIDASE BY TRICHODERMA-VIRIDE ITCC-1433 IN SUBMERGED CULTURE ON DIFFERENT SUBSTRATES
SECRETION OF CELLULASE AND BETA-GLUCOSIDASE BY TRICHODERMA-VIRIDE ITCC-1433 IN SUBMERGED CULTURE ON DIFFERENT SUBSTRATES
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DOI：
10.1002/bit.260210805
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发表时间：
1979-01-01
影响因子：
3.8
通讯作者：
HERR, D
中科院分区：
文献类型：
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作者：
HERR, D
T. viride ITCC-1433产生高产的纤维素酶，特别是β-葡萄糖苷酶在不同C源的深层培养中生长的差异。纤维素酶合成在葡萄糖存在下被强烈抑制，并且β-葡萄糖的组成活性仅低。在培养T.绿色葡萄糖。羧甲基纤维素（CMC）作为底物的酶的分泌和生长依赖于取代度，但在一般情况下，CMC不能被视为一个强大的诱导剂或作为C源。与不溶性纤维素，最大的酶的生产和活动，获得使用碱处理的纤维素粉末。在此底物上，分泌到培养液中的可溶性蛋白质增加，并且蛋白质浓度对应于纤维素分解活性。
T. viride ITCC-1433 produces high yields of cellulase and especially .beta.-glucosidase when grown in submerged culture on different C sources. Cellulase synthesis was strongly repressed in the presence of glucose and only a low constitutive activity of .beta.-glucosidase and carboxymethylcellulase, but no Avicelase, could be demonstrated when culturing T. viride on glucose. With carboxymethylcellulose (CMC) as a substrate the secretion of enzyme and growth depended on the degree of substitution, but in general CMC cannot be regarded either as a powerful inducer or as a C source. With insoluble cellulose, maximum enzyme production and activities were obtained using an alkali-treated cellulose powder. On this substrate the excretion of soluble protein into the culture broth increased and the protein concentration corresponded to cellulolytic activities.