Defensive Role of Tomato Polyphenol Oxidases against Cotton Bollworm (Helicoverpa armigera) and Beet Armyworm (Spodoptera exigua)
Defensive Role of Tomato Polyphenol Oxidases against Cotton Bollworm (Helicoverpa armigera) and Beet Armyworm (Spodoptera exigua)
复制标题
DOI：
10.1007/s10886-008-9571-7
复制
发表时间：
2009-01-01
影响因子：
2.3
通讯作者：
Tantasawat, Piyada
中科院分区：
文献类型：
--
作者：
Bhonwong, Anongnut;Stout, Michael J.;Tantasawat, Piyada
番茄多酚氧化酶（Polypolyphenoxidases，PPO）是一种将酚类物质氧化为醌类物质的酶，与植物抗虫性有关。研究了多酚氧化酶（PPO）在棉铃虫（Helicoverpa armigera（Hubner））和甜菜夜蛾（Spodoptera exigua（Hubner））抗性中的作用。消费，体重增加，和幼虫取食过表达PPO的转基因番茄系（OP系）和幼虫取食抑制PPO的转基因番茄系（SP系）的叶子的死亡率进行了比较，消费，体重增加，和幼虫取食非转化（NT）植物的死亡率。与NT和OP植物相比，观察到以SP植物的叶子为食的棉铃虫的叶子消耗和重量增加。PPO活性与棉铃虫的增重和取食量呈负相关，说明PPO对棉铃虫具有防御作用。类似地，甜菜夜蛾从OP植物比SP植物消耗更少的叶子（幼叶和老叶两者）。幼虫取食OP叶一般表现出较低的增重比SP叶。这些结果表明，番茄多酚氧化酶在抗棉铃虫和甜菜夜蛾中起作用。
Tomato (Solanum lycopersicum) polyphenol oxidases (PPOs), enzymes that oxidize phenolics to quinones, have been implicated in plant resistance to insects. The role of PPO in resistance to cotton bollworm [Helicoverpa armigera (Hubner)] and beet armyworm [Spodoptera exigua (Hubner)] (Lepidoptera: Noctuidae) was evaluated. Consumption, weight gains, and mortality of larvae feeding on foliage of transgenic tomato lines overexpressing PPO (OP lines) and of larvae feeding on foliage of transgenic tomato lines with suppressed PPO (SP lines) were compared with consumption, weight gains, and mortality of larvae feeding on non-transformed (NT) plants. Increases in foliage consumption and weight gains were observed for cotton bollworms feeding on leaves of SP plants compared to NT and OP plants. PPO activity was negatively correlated with both weight gains and foliar consumption of cotton bollworm, substantiating the defensive role of PPO against this insect. Similarly, beet armyworm consumed less foliage (both young and old leaves) from OP plants than SP plants. Larvae feeding on OP leaves generally exhibited lower weight gains than those feeding on SP leaves. These results indicate that tomato PPO plays a role in resistance to both cotton bollworm and beet armyworm.