Collagen-Coated Poly(L-lactide-co-ε-caprolactone) Film: A Promising Scaffold for Cultured Periosteal Sheets
Collagen-Coated Poly(L-lactide-co-ε-caprolactone) Film: A Promising Scaffold for Cultured Periosteal Sheets
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DOI：
10.1902/jop.2010.100194
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发表时间：
2010-11-01
影响因子：
4.3
通讯作者：
Yoshie, Hiromasa
中科院分区：
文献类型：
--
作者：
Kawase, Tomoyuki;Yamanaka, Katsuyuki;Yoshie, Hiromasa
背景资料：我们以前证明，人骨膜片培养皿上制备的功能作为成骨“移植材料”适用于牙周再生治疗。然而，切下的骨膜组织段与培养皿的初始粘附水平较低是影响功能性骨膜片成功制备的关键点。为了改善这一弱点，我们开发了一种透明的，可生物降解的聚（L-丙交酯-co-ε-己内酯）（LCL）膜，并测试其功能作为支架和载体的骨膜sheets.Methods：人骨膜组织段切离健康供体的牙槽骨培养I型atelocollagen-coated LCL膜。通过简单搅拌检查初始粘附力。细胞生长和体外矿化进行细胞组织化学检查。成骨活性进行组织化学检查，在动物植入模型中使用nude mice.Results：表面胶原涂层改性LCL的疏水性，并大大提高了初始粘附。与塑料培养皿中的培养物相比，无涂层膜中的生长速率延迟，但胶原涂层膜中的生长速率未延迟。在动物植入的修剪过程中，骨膜片经常与非涂层膜分离，但不会与胶原涂层膜分离。无论胶原涂层，LCL电影并没有造成任何显着的浸润炎性细胞，或产生负面影响矿化tissueformation.Conclusions：胶原涂层提高了骨膜段的初始粘附，这有利于细胞生长，也处理效率植入。因此，我们相信，一旦在人体研究中进行评估，我们的胶原蛋白涂层LCL膜将有助于改善牙周再生方法与培养自体骨膜片的应用。J Periodontol 2010;81：1653-1662.
Background: We previously demonstrated that human periosteal sheets prepared on culture dishes function as an osteogenic "graft material" applicable to periodontal regenerative therapy. However, a lower level of initial adhesion of the excised periosteum tissue segments to culture dishes was a critical point that compromised the successful preparation of functional periosteal sheets. To improve on this weakness, we developed a transparent, biodegradable poly(L-lactide-co-epsilon-caprolactone) (LCL) film and tested its function as a scaffold and carrier of periosteal sheets.Methods: Human periosteum tissue segments excised from alveolar bone of healthy donors were cultured on type I atelocollagen-coated LCL films. Initial adhesion was examined by simple agitation. Cell outgrowth and in vitro mineralization were cytohistochemically examined. Osteogenic activity was histochemically examined in an animal implantation model using nude mice.Results: Surface collagen-coating modified the hydrophobic nature of LCL and substantially improved the initial adhesion. Compared to cultures in plastic dishes, the growth rate was delayed in non-coated films, but not in collagen-coated films. In the trimming process for animal implantation, periosteal sheets were frequently detached from non-coated films, but not from collagen-coated films. Regardless of collagen-coating, LCL films did not cause any significant infiltration of inflammatory cells, or negatively impact mineralized tissue formation.Conclusions: Collagen-coating improved the initial adhesion of periosteum segments, which facilitated cell outgrowth and also handling efficiency on implantation. Therefore, we believe that once evaluated in human studies, our collagen-coated LCL film will contribute to improving the periodontal regenerative methodology with the application of cultured autologous periosteal sheets. J Periodontol 2010;81:1653-1662.