A Polyclonal Antibody Against Active C-Terminal ADAMTS-18 Fragment
A Polyclonal Antibody Against Active C-Terminal ADAMTS-18 Fragment
复制标题
抗活性 C 端 ADAMTS-18 片段的多克隆抗体
DOI：
10.1089/hyb.2011.0055
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发表时间：
2011-12-01
期刊：
影响因子：
--
通讯作者：
Zhang, Wei
中科院分区：
文献类型：
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作者：
Dang, Suying;Bu, Dawei;Zhang, Wei
The human ADAMTS-18, a disintegrin and metalloproteinase with thrombospondin type-1 modules 18, is a secreted Zn-metalloproteinase. The C-terminal 385-amino acid fragment of ADAMTS-18 (AD18C) is highly effective at promoting platelet thrombus dissolution in vivo. Therefore, polyclonal antibody (pAb) against AD18C fragment should be able to keep platelet thrombus stability, which has direct clinical relevance. In this report, pAb against AD18C fragment was generated from rabbit immunized with AD18C recombinant protein (rAD18C). The pAb showed specific binding with rAD18C and natural ADAMTS-18 protein by ELISA and Western blot assay. It shortens the mouse tail bleeding time in a dose-dependent manner. Thus, anti-AD18C pAb contributes to the regulation of platelet thrombus stability.
