Symmetry and connectivity in the map of the body surface in somatosensory area II of primates.
Symmetry and connectivity in the map of the body surface in somatosensory area II of primates.
复制标题
灵长类动物体感区域 II 体表图的对称性和连通性。
DOI：
10.1152/jn.1969.32.2.170
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发表时间：
1969
影响因子：
2.5
通讯作者：
G. Werner
中科院分区：
文献类型：
--
作者：
B. Whitsel;L. Petrucelli;G. Werner
方法实验在16只猕猴身上进行。在氟烷麻醉下进行实验之前的外科手术。这些程序包括在颅骨开口上植入记录室、闭合肌肉和皮肤切口、浸润和表面麻醉以及气管插管。由于引言中所述的原因，有必要在没有全身麻醉的情况下研究SII中神经元的反应：因此，在开始电记录前1小时停止氟烷给药。为了避免刺痛，我们没有使用耳杆或其他设备进行头部固定，这会对软组织造成压力;相反，我们通过将植入的记录室固定到一个框架上来支撑头部，该框架也带有微驱动器。为防止实验制剂出现任何不适而采取的额外预防措施，以及使我们确信这些预防措施有效性的证据，已在以前的出版物中进行了描述（39，40）。在所有实验中，均使用三乙基碘化没食子胺维持神经肌肉阻滞，并调整通气速率和通气量，使潮气末CO维持在4%。皮肤和直肠温度也保持在正常值。采用玻璃绝缘钨微电极（39）通过充满人工脑脊髓液（12）的封闭室进行细胞外记录。电极通过校准的微螺钉穿过皮质，为了能够精确重建电极轨迹，通过使直流电通过电极，在每次穿透的末端位置形成损伤。5组电流范围为1 -5 pa，通常产生的损伤在组织切片中很容易识别（见图1）。我们的刺激和记录程序的细节已经发表在以前（39，40）。
METHODSThe experiments were carried out in 16 macaques. The surgical procedures which preceded the experiments were performed under halothane anesthesia. These procedures consisted of implantation of a recording chamber over an opening in the skull, closure of the muscles and skin incisions, infiltration and topical anesthesia, and tracheal intubation. For reasons stated in the introduction, it was necessary to study the responres of neurons in SII in the absence of general anesthesia: therefore, halothane administration was discontinued 1 hr prior to the start of the electrical recording. To avoid in-Hicting pain, we did not use ear bars or other devices for head fixation which cause pressure on soft tissues; instead we supported the head by fixing the implanted recording chamber to a frame which also carried the microdrive. The additional precautions taken to prevent any discomfort of the experimental preparations, and the evidence that reassured us of the effectiveness of these precautions, have been described in a previous publication(39, 40). Neuromuscular block was maintained in all experiments with gallamine triethiodide, and the ventilation rate and volume were adjusted so that the end-tidal CO, was maintained at 4%. The skin and rectal temperatures were also maintained at their normal values. Glass-insulated tungsten microelectrodes(39) were employed for extracellular recording through a closed chamber filled with artificial cerebrospinal fluid (12). The electrode was advanced through the cortex by a calibrated microscrew.To enable accurate reconstruction of the electrode tracks, a lesion was made at the terminal position of each penetration by passing direct current through the electrode. Currents ranging from l-5 pa for 5 set usually produced lesions that were easily recognizable in the histological sections (see Fig. 1). The details of our stimulating and recording procedures have been published previously(39, 40).