Localization of collagen types I, III and V during tendon development. Changes in collagen types I and III are correlated with changes in fibril diameter.
Localization of collagen types I, III and V during tendon development. Changes in collagen types I and III are correlated with changes in fibril diameter.
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DOI：
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发表时间：
1997-04
影响因子：
6.6
通讯作者：
D. Birk;R. Mayne
中科院分区：
文献类型：
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作者：
D. Birk;R. Mayne
I型、III型和V型胶原位于肌腱发育的不同阶段：肌腱结构建立但不成熟（14天）的阶段、成熟肌腱（孵化）和肌腱原纤维快速生长的中间点（17天）。研究了腱束及其结缔组织包埋物（endotendenium）。这些数据显示了在发育中的跖骨肌腱中III型胶原蛋白表达的变化模式。在发育14天的未成熟肌腱中，I型和III型胶原共分布于整个肌束及其结缔组织投资中。在这个阶段，所有的原纤维，无论网站是小的。随着发展，这些地区分离开来，变得很容易辨认。当这种情况发生时，纤维直径分布发散;那些在束变得更大，而那些在endendendenium保持小。在此期间，纤维束失去III型胶原蛋白表达，而腱内膜变得富含III型胶原蛋白。与此同时，结缔组织的投资发展，和纤维的endendendenium保持小，在此期间，但随后变得更大的成熟肌腱。直径的增加与III型胶原蛋白反应性的降低有关。在孵化时，显著的III型胶原蛋白反应性和小直径原纤维都局限于外鞘。在肌腱发育的所有阶段，有一个恒定的小，但可检测到的量的V型胶原蛋白。然而，没有V型反应性和原纤维直径之间的相关性，观察在任何阶段的发展。这些结果表明，III型胶原蛋白的反应性和原纤维直径在发展肌腱之间的反比关系。
Collagen types I, III and V were localized at different stages of tendon development: a stage when tendon architecture is established, but immature (14-day), a mature tendon (hatchling) and an intermediate point where there is a rapid growth of tendon fibrils (17-day). The tendon fascicles and their connective tissue investments (endotendenium) were studied. The data show a changing pattern of type III collagen expression in the developing metatarsal tendon. In the immature tendon at 14 days of development, collagen types I and III are codistributed throughout the fascicles and their connective tissue investments. At this stage all of the fibrils regardless of the site are small. With development the regions segregate and become easily recognizable. As this occurs, the fibril diameter distributions diverge; those in the fascicle become larger while those in the endotendenium remain small. During this period, the fascicle loses type III collagen expression, while the endotendenium becomes type III collagen rich. At the same time, the connective tissue investments develop, and the fibrils of the endotendenium remain small during this period, but then become larger in the mature tendon. The increases in diameter are associated with a decrease in type III collagen reactivity. At hatching, both significant collagen type III reactivity and small diameter fibrils are restricted to the outer sheaths. During all stages of tendon development there is a constant small, but detectable amount of type V collagen. However, no correlation between type V reactivity and fibril diameter was observed at any stage of development. These results indicate an inverse relationship between type III collagen reactivity and fibril diameter in the developing tendon.