Proteomics profiling of interactome dynamics by colocalisation analysis (COLA).
Proteomics profiling of interactome dynamics by colocalisation analysis (COLA).
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DOI：
10.1039/c6mb00701e
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发表时间：
2016-12-20
影响因子：
--
通讯作者：
Bakal C
中科院分区：
文献类型：
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作者：
Mardakheh FK;Sailem HZ;Kümper S;Tape CJ;McCully RR;Paul A;Anjomani-Virmouni S;Jørgensen C;Poulogiannis G;Marshall CJ;Bakal C
蛋白质组学和生物信息学相结合的策略，通过评估亚细胞蛋白质-蛋白质共定位来绘制相互作用组动力学。在真核生物中，定位和蛋白质功能密切相关，因为蛋白质定位于特定的区室，在那里它们与其他功能相关的蛋白质接近。因此，两种蛋白质的显着共定位可以表明它们的功能关联。我们在此介绍 COLA，这是一种基于蛋白质组学的策略，结合生物信息学框架，可在全球范围内检测蛋白质-蛋白质共定位。 COLA 通过匹配亚细胞定位特征显着相似的蛋白质来揭示功能相互作用。 COLA 的快速特性允许高精度地绘制不同条件或治疗下的相互作用组动力学图谱。
A combined proteomics and bioinformatics strategy for mapping interactome dynamics via assessment of subcellular protein–protein colocalisations. Localisation and protein function are intimately linked in eukaryotes, as proteins are localised to specific compartments where they come into proximity of other functionally relevant proteins. Significant co-localisation of two proteins can therefore be indicative of their functional association. We here present COLA, a proteomics based strategy coupled with a bioinformatics framework to detect protein–protein co-localisations on a global scale. COLA reveals functional interactions by matching proteins with significant similarity in their subcellular localisation signatures. The rapid nature of COLA allows mapping of interactome dynamics across different conditions or treatments with high precision.