Epidermal growth factor binding sites in human pituitary macroadenomas
Epidermal growth factor binding sites in human pituitary macroadenomas
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DOI：
10.1677/joe.0.1580425
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发表时间：
1998-09-01
影响因子：
4
通讯作者：
Gulino, A
中科院分区：
文献类型：
--
作者：
Jaffrain-Rea, ML;Petrangeli, E;Gulino, A
表皮生长因子（EGF）结合位点的数量进行了测定，在一系列的46垂体大腺瘤的竞争性结合试验。单一浓度的I-125-EGF（1 nM）用于所有实验。在四种情况下，通过加入增加浓度的冷EGF获得置换曲线，Scatchard分析显示存在两类EGF结合位点，高亲和力和低亲和力结合位点的K-d1 = 0.62 +/- 0.23 nM和K-d2 = 53.8 +/- 8.2 nM。EGF结合位点的分布进行了研究，在42例单点测定，在存在和不存在的100倍的冷EGF过量。观察到EGF结合位点的非参数分布（中位数10.2 fmol/mg膜蛋白，范围0.0-332.0）。EGF受体阳性，定义为EGF结合大于或等于10.0 fmol/mg蛋白，在23例（54.8%）中观察到，尤其是在泌乳素瘤中（76.5%，P
The number of epidermal growth factor (EGF) binding sites was determined by competitive binding assays in a series of 46 pituitary macroadenomas. A single concentration of I-125-EGF (1 nM) was used for all experiments. In four cases, a displacement curve was obtained by adding increasing concentrations of cold EGF, and Scatchard analysis showed the presence of two classes of EGF binding sites, with K-d1 = 0.62 +/- 0.23 nM and K-d2 = 53.8 +/- 8.2 nM for the high- and low-affinity binding sites respectively. The distribution of EGF binding sites was studied in 42 cases by a single-point assay, in the presence and in the absence of a 100-fold cold EGF excess. A non-parametric distribution of EGF binding sites was observed (median 10.2 fmol/mg membrane protein, range 0.0-332.0). EGF-receptor positivity, defined as EGF binding greater than or equal to 10.0 fmol/mg protein, was observed in 23 samples (54.8%), especially in prolactinomas (76.5%, P