Virulence of major periodontal pathogens and lack of humoral immune protection in a rat model of periodontal disease.
Virulence of major periodontal pathogens and lack of humoral immune protection in a rat model of periodontal disease.
复制标题
牙周病大鼠模型中主要牙周病原体的毒力和体液免疫保护的缺乏。
DOI：
10.1111/j.1601-0825.2010.01678.x
复制
发表时间：
2010
期刊：
影响因子：
3.8
通讯作者：
Kesavalu,L
中科院分区：
文献类型：
--
作者：
Verma,RK;Bhattacharyya,I;Sevilla,A;Lieberman,I;Pola,S;Nair,M;Wallet,SM;Aukhil,I;Kesavalu,L
Oral Diseases（2010）16，686- 695目的：本研究旨在使用大鼠中混合微生物感染的模型来测试牙周病原体Tannerella gingivalis和Porphyromonas gingivalis在毒力潜力方面是协同的假设。将单菌感染或混合微生物感染中的牙龈卟啉单胞菌或牙龈卟啉单胞菌感染12周，并将假感染组用作对照。 本研究探讨了细菌感染，炎症，免疫，牙槽骨丢失的变化与疾病progress.Results的变化：Tannerella dangerthia和牙龈卟啉单胞菌基因组DNA从感染大鼠的微生物样品中检测到PCR表明，它们在大鼠口腔定植。初次感染诱导显著高的IgG、IgG 2b、IgG 1和IgG 2a抗体水平，表明混合Th 1和Th 2免疫应答的激活。感染混合微生物菌群的大鼠表现出显著增加的腭水平和邻间牙槽骨丢失。组织学检查显示牙龈上皮明显增生，伴有中度炎症浸润和结合上皮顶端迁移。结论：本研究结果表明，T。牙龈卟啉单胞菌和P. gingivalis表现出毒力，但没有毒力协同作用，导致大鼠牙周炎期间的免疫炎症反应和缺乏体液免疫保护。
Oral Diseases(2010)16, 686–695Objective:This study was designed to test the hypothesis that periodontal pathogens Tannerella forsythia and Porphyromonas gingivalis are synergistic in terms of virulence potential using a model of mixed‐microbial infection in rats.Materials and methods:Three groups of rats were infected orally with either T. forsythia or P. gingivalis in mono‐bacterial infections or as mixed‐microbial infections for 12 weeks and a sham‐infected group were used as a control. This study examined bacterial infection, inflammation, immunity, and alveolar bone loss changes with disease progression.Results:Tannerella forsythia and P. gingivalis genomic DNA was detected in microbial samples from infected rats by PCR indicating their colonization in the rat oral cavity. Primary infection induced significantly high IgG, IgG2b, IgG1, and IgG2a antibody levels indicating activation of mixed Th1 and Th2 immune responses. Rats infected with the mixed‐microbial consortium exhibited significantly increased palatal horizontal and interproximal alveolar bone loss. Histological examinations indicated significant hyperplasia of the gingival epithelium with moderate inflammatory infiltration and apical migration of junctional epithelium. The results observed differ compared to uninfected controls.Conclusion:Our results indicated that T. forsythia and P. gingivalis exhibit virulence, but not virulence synergy, resulting in the immuno‐inflammatory responses and lack of humoral immune protection during periodontitis in rats.