RSC1 and RSC2 are required for expression of mid-late sporulation-specific genes in Saccharomyces cerevisiae
RSC1 and RSC2 are required for expression of mid-late sporulation-specific genes in Saccharomyces cerevisiae
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DOI：
10.1128/ec.3.4.910-918.2004
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发表时间：
2004-08-01
期刊：
影响因子：
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通讯作者：
Winter, E
中科院分区：
文献类型：
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作者：
Bungard, D;Reed, M;Winter, E
RSC1和RSC2是酿酒酵母中依赖于三磷酸腺苷的RSC染色质重塑复合体的选择性含溴结构域的亚基。Smk1是一种孢子形成特异性的有丝分裂原激活的蛋白激酶同系物，是孢子形成的减数分裂后事件所必需的。在这项研究中，我们发现RSC1和RSC2是单倍体不足以在库中形成低晶型的孢子。此外，缺乏Rsc1或Rsc2的二倍体表现出类似smk1的表型子集。高拷贝数的RSC1质粒不抑制RSC2-Delta/RSC2-Delta孢子形成缺陷，高拷贝数的RSC2质粒不抑制rsc1-Delta/rsc1-Delta孢子形成缺陷。在缺乏Rsc1或Rsc2的细胞中，通常在孢子组装的关键步骤发生时表达的中晚期产孢特异性基因，包括形成孢子壁所需的基因，不表达。我们推测，RSC1和RSC2在中晚期启动子的联合作用对于这组独特的定时基因的正确表达是特别必要的。我们的数据表明，Smk1和Rsc1/2定义了平行的通路，它们汇聚在一起，分别提供孢子形态发生所需的信号信息和基因产物的表达。
Rsc1 and Rsc2 are alternative bromodomain-containing subunits of the ATP-dependent RSC chromatin remodeling complex in Saccharomyces cerevisiae. Smk1 is a sporulation- specific mitogen-activated protein kinase homolog that is required for the postmeiotic events of spore formation. In this study we show that RSC1 and RSC2 are haploinsufficient for spore formation in a sink] hypomorph. Moreover, diploids lacking Rsc1 or Rsc2 show a subset of smk1-like phenotypes. High-copy-number RSC1 plasmids do not suppress rsc2-Delta/rsc2-Delta sporulation defects, and high-copy-number RSC2 plasmids do not suppress rsc1-Delta/rscl-Delta sporulation defects. Mid-late sporulation-specific genes, which are normally expressed while key steps in spore assembly occur and which include genes that are required for spore wall formation, are not expressed in cells lacking Rsc1 or Rsc2. We speculate that the combined action of Rsc1 and Rsc2 at mid-late promoters is specifically required for the proper expression of this uniquely timed set of genes. Our data suggest that Smk1 and Rsc1/2 define parallel pathways that converge to provide signaling information and the expression of gene products, respectively, that are required for spore morphogenesis.