Expression of β-defensins in intestines of chickens injected with vitamin D3 and lipopolysaccharide
Expression of β-defensins in intestines of chickens injected with vitamin D3 and lipopolysaccharide
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DOI：
10.4238/2015.april.13.12
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发表时间：
2015-01-01
影响因子：
0.4
通讯作者：
Liu, Y. P.
中科院分区：
文献类型：
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作者：
Lu, L.;Li, S. M.;Liu, Y. P.
本研究的目的是评估维生素D-3（VD 3）对正常和脂多糖（LPS）条件下鸡肠道β-防御素基因的调节作用。使用四个处理组，包括阴性对照组、VD 3注射组、LPS注射组以及VD 3注射和LPS注射组。在注射后4、24和48 h，收集肠道并分离RNA，以使用定量聚合酶链反应测量具有推定的维生素D响应元件的鸡β-防御素基因。所有7个鸡β-防御素基因的表达在肠道中是可检测的。在注射LPS后4、24和48 h分别发现GAL-6、GAL-7和GAL-9的显著增加，而在正常条件下VD 3注射不影响任何研究的基因的表达。然而，GAL-4、GAL-5、GAL-6和GAL-10的表达被VD 3与LPS组合协同上调。这些结果表明，VD 3通过诱导鸡β-防御素基因的表达，增强了免疫应激过程中的免疫力。
The objective of this study was to evaluate the effect of vitamin D-3 (VD3) on the regulation of chicken intestinal beta-defensin genes under normal and lipopolysaccharides (LPS) conditions. Four treatment groups were used, including a negative control group, VD3-injection group, LPS-injection group, and both VD3-injection and LPS-injection group. At 4, 24, and 48 h post-injection, intestines were collected and RNA was isolated to measure the chicken beta-defensin genes with putative vitamin D responsive elements using quantitative polymerase chain reaction. Expressions of all 7 chicken beta-defensin genes was detectable in the intestines. Significant increases in GAL-6, -7 and -9 were found following LPS injection treatment at 4, 24, and 48 h post-injection, respectively, whereas VD3 injection did not affect the expression of any investigated genes under normal conditions. However, the expression of GAL-4, -5, -6, and -10 were synergistically upregulated by VD3 in combination with LPS. These results suggest that VD3 enhances the immune immunity during LPS challenge by inducing the expression of chicken beta-defensin genes when birds are exposed to immune stressors.