Similarities in the repair kinetics of sublethal and potentially lethal X-ray damage in log phase Chinese hamster V79 cells.
Similarities in the repair kinetics of sublethal and potentially lethal X-ray damage in log phase Chinese hamster V79 cells.
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对数期中国仓鼠 V79 细胞亚致死和潜在致死 X 射线损伤修复动力学的相似性。
DOI：
10.1080/09553008914551411
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发表时间：
1989
影响因子：
2.6
通讯作者：
Lange,CS
中科院分区：
文献类型：
--
作者：
Reddy,NM;Lange,CS
我们比较了V79细胞在37℃培养条件下，X射线亚致死损伤(SLD)和潜在致死损伤(PLD)的修复动力学。用剂量分级法研究了SLD修复的动力学。照射后不同时间用0.5M高渗盐水孵育细胞20min，研究PLD修复的动力学。2Gy4和10GySLD修复时间分别为11min、23min和62min。该修复时间与剂量D2(测试剂量)的大小无关。2、4和10GyPLD的修复时间分别为9、23和61min。这些数据表明：(1)给定剂量下SLD的修复动力学与测试剂量D2的大小无关；(2)完成修复所需的时间(修复时间)取决于剂量d1，即剂量d1越大，修复时间越长；(3)对于给定剂量，通过剂量分段或HS处理测定的修复时间相似；(4)修复时间的相似性可能意味着SLD和PLD是相同的。在分段生存曲线上，讨论了修复动力学与肩部形状的关系。
We have compared the repair kinetics of X-ray-induced sublethal damage (SLD) and potentially lethal damage (PLD) in V79 cells during postirradiation incubation in growth medium at 37°C. Kinetics of SLD repair were studied by dose fractionation (D1 + D2 = 2 + 2, 4 + 2, 4 + 4, 10 + 4 and 10 + 7 Gy). Kinetics of PLD repair were studied by incubating cells with 0·5mhypertonic saline (HS) for 20 min at various times after irradiation. The repair of SLD, after 2, 4 and 10 Gy, was complete in about 11, 23 and 62 min, respectively. This repair time was independent of the magnitude of dose D2 (test dose). The repair of PLD, after 2, 4 and 10 Gy, was also complete in about 9, 23 and 61 min, respectively. These data indicate that: (1) the kinetics of repair of SLD for a given dose are independent of the magnitude of the test dose D2; (2) the time taken to complete the repair (repair time) is dependent on dose D1, i.e. the larger the dose D1 the longer the repair time; (3) for a given dose, the repair times assayed either by dose fractionation or by HS treatment are similar; and (4) similarities in the repair times may imply that SLD and PLD are the same. The relationship between the repair kinetics and the shape of the shoulder on fractionated survival curves is discussed.
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影响因子：
1.7
作者：
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影响因子：
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影响因子：
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影响因子：
3.4
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通讯作者：
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