Investigating the role of polyols in Cladosporium fulvum during growth under hyper-osmotic stress and in planta
Investigating the role of polyols in Cladosporium fulvum during growth under hyper-osmotic stress and in planta
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DOI：
10.1007/s00425-002-0833-2
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发表时间：
2003-02-01
期刊：
影响因子：
4.3
通讯作者：
Oliver, RP
中科院分区：
文献类型：
--
作者：
Clark, AJ;Blissett, KJ;Oliver, RP
番茄真菌病原体黄枝孢霉（Cladosporium fulvum）（syn. Fulvia fulva，Cooke）在植物和无菌培养物中的生长情况进行了研究。在体外高渗胁迫下，阿拉伯糖醇和甘油积累。甘露醇水平较低，在高浓度胁迫的菌丝体。C-13 NMR光谱表明，在高渗条件下，从葡萄糖到甘露醇的碳流被重定向到阿拉伯糖醇和甘油。番茄（Lycopersicon esculentum Mill.）植物含有所有三种多元醇，而甘油是未感染植物中存在的唯一多元醇，这表明甘露醇和阿拉伯糖醇是真菌来源的。与充分浇水的植物相比，受限制浇水的受感染植物中存在显著更高水平的阿拉伯糖醇和甘油。结果表明，真菌阿拉伯糖醇和甘油，而不是甘露醇，主要作用是调节和水的收购是致病性的一个重要方面。
The role of the large amounts of polyols accumulated by the fungal tomato pathogen, Cladosporium fulvum (syn. Fulvia fulva, Cooke) both in planta and in axenic cultures has been examined. Arabinitol and glycerol accumulated in response to hyper-osmotic stress in vitro. Mannitol levels were lower in osmo-stressed mycelium. C-13 NMR spectroscopy indicated that carbon flow from glucose to mannitol was redirected to arabinitol and glycerol in hyper-osmotic conditions. Infected tomato (Lycopersicon esculentum Mill.) plants contained all three polyols whereas glycerol was the only polyol present in uninfected plants, suggesting that the mannitol and arabinitol were of fungal origin. Substantially higher levels of arabinitol and glycerol were present in infected plants that were subjected to a restricted watering regime compared to fully watered plants. The results suggest that a primary role of fungal arabinitol and glycerol, but not mannitol, is osmoregulation and that water acquisition is an important aspect of pathogenicity.